Bone marrow Derived Pluripotent Cells are Pericytes which Contribute to Vascularization

Cai, Xiaoxiao; Lin, Yunfeng; Friedrich, Claudia C; Neville, Craig; Pomerantseva, Irina; Sundback, Cathryn A; Sharma, Parul; Zhang, Zhiyuan; Vacanti, Joseph P; Hauschka, Peter V; Grottkau, Brian E

doi:10.1007/s12015-009-9097-6

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Cai, X., Lin, Y., Friedrich, C. C., Neville, C., Pomerantseva, I., Sundback, C. A., Sharma, P., Zhang, Z., Vacanti, J. P., Hauschka, P. V., & Grottkau, B. E. (2009). Bone marrow Derived Pluripotent Cells are Pericytes which Contribute to Vascularization. Stem Cell Reviews, 5(4), 437–445. https://doi.org/10.1007/s12015-009-9097-6

Abstract

Pericytes are essential to vascularization, but the purification and characterization of pericytes remain unclear. Smooth muscle actin alpha (α-SMA) is one maker of pericytes. The aim of this study is to purify the α-SMA positive cells from bone marrow and study the characteristics of these cells and the interaction between α-SMA positive cells and endothelial cells. The bone marrow stromal cells were harvested from α-SMA-GFP transgenic mice, and the α-SMA-GFP positive cells were sorted by FACS. The proliferative characteristics and m