Publication: Absolute quantification of transcription factors during cellular differentiation using multiplexed targeted proteomics
Absolute quantification of transcription factors during cellular differentiation using multiplexed targeted proteomics
Date
Date
Date
Citations
Simicevic, J., Schmid, A. W., Gilardoni, P. A., Zoller, B., Raghav, S. K., Krier, I., Gubelmann, C., Lisacek, F., Naef, F., Moniatte, M., & Deplancke, B. (2013). Absolute quantification of transcription factors during cellular differentiation using multiplexed targeted proteomics. Nature Methods, 10, 570–576. https://doi.org/10.1038/nmeth.2441
Abstract
Abstract
Abstract
The cellular abundance of transcription factors (TFs) is an important determinant of their regulatory activities. Deriving TF copy numbers is therefore crucial to understanding how these proteins control gene expression. We describe a sensitive selected reaction monitoring–based mass spectrometry assay that allowed us to determine the copy numbers of up to ten proteins simultaneously. We applied this approach to profile the absolute levels of key TFs, including PPARɣ and RXRα, during terminal differentiation of mouse 3T3-L1 pre-adipoc
Additional indexing
Creators (Authors)
- Simicevic, Jovan
- Schmid, Adrien W
- Gilardoni, Paola A
- Zoller, Benjamin
- Raghav, Sunil K
- Krier, Irina
- Gubelmann, Carinne
- Lisacek, Frédérique
- Naef, Felix
- Moniatte, Marc
- Deplancke, Bart
Volume
Volume
Volume
Number
Number
Number
Page range/Item number
Page range/Item number
Page range/Item number
Page end
Page end
Page end
Item Type
Item Type
Item Type
Language
Language
Language
Publication date
Publication date
Publication date
Date available
Date available
Date available
ISSN or e-ISSN
ISSN or e-ISSN
ISSN or e-ISSN
OA Status
OA Status
OA Status
Free Access at
Free Access at
Free Access at
Publisher DOI
Citations
Simicevic, J., Schmid, A. W., Gilardoni, P. A., Zoller, B., Raghav, S. K., Krier, I., Gubelmann, C., Lisacek, F., Naef, F., Moniatte, M., & Deplancke, B. (2013). Absolute quantification of transcription factors during cellular differentiation using multiplexed targeted proteomics. Nature Methods, 10, 570–576. https://doi.org/10.1038/nmeth.2441
