Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

López-Labrador, F Xavier; Brown, Julianne R; Fischer, Nicole; Harvala, Heli; Van Boheemen, Sander; Cinek, Ondrej; Sayiner, Arzu; Madsen, Tina Vasehus; Auvinen, Eeva; Kufner, Verena; Huber, Michael; Rodriguez, Christophe; Jonges, Marcel; Hönemann, Mario; Susi, Petri; Sousa, Hugo; Klapper, Paul E; Pérez-Cataluňa, Alba; Hernandez, Marta; Molenkamp, Richard

doi:10.1016/j.jcv.2020.104691

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López-Labrador, F. X., Brown, J. R., Fischer, N., Harvala, H., Van Boheemen, S., Cinek, O., Sayiner, A., Madsen, T. V., Auvinen, E., Kufner, V., Huber, M., Rodriguez, C., Jonges, M., Hönemann, M., Susi, P., Sousa, H., Klapper, P. E., Pérez-Cataluňa, A., Hernandez, M., … et al. (2021). Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure. Journal of Clinical Virology, 134, 104691. https://doi.org/10.1016/j.jcv.2020.104691

Abstract

Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring togethe