Publication: Isolation and Analysis of MicroRNAs from Extracellular Vesicles of the Parasitic Model Nematodes Nippostrongylus brasiliensis and Trichuris muris
Isolation and Analysis of MicroRNAs from Extracellular Vesicles of the Parasitic Model Nematodes Nippostrongylus brasiliensis and Trichuris muris
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Eichenberger, R. M. (2021). Isolation and Analysis of MicroRNAs from Extracellular Vesicles of the Parasitic Model Nematodes Nippostrongylus brasiliensis and Trichuris muris. In L. M. de Pablos & J. Sotillo (Eds.), Parasite Genomics : Methods and Protocols (pp. 319–332). Springer. https://doi.org/10.1007/978-1-0716-1681-9_17
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The identification, detection, and use of small RNA species have rapidly gained interest—especially to study parasite–host interactions. Parasite-to-host communication is contributed by small secreted extracellular vesicle (EV)-derived nucleic acid species. In particular, microRNAs (miRNAs) and small interfering RNAs can regulate the host response by targeting cells at both transcriptional and posttranscriptional levels. Here, modified protocols for density gradient purification of EVs from nematodes and the subsequent extraction of E
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Eichenberger, R. M. (2021). Isolation and Analysis of MicroRNAs from Extracellular Vesicles of the Parasitic Model Nematodes Nippostrongylus brasiliensis and Trichuris muris. In L. M. de Pablos & J. Sotillo (Eds.), Parasite Genomics : Methods and Protocols (pp. 319–332). Springer. https://doi.org/10.1007/978-1-0716-1681-9_17