Publication: A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity
A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity
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Sheppard, E. C., Rogers, S., Harmer, N. J., & Chahwan, R. (2019). A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity. Scientific Reports, 9(1), 8853. https://doi.org/10.1038/s41598-019-45356-z
Abstract
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Abstract
DNA and RNA nucleases play a critical role in a growing number of cellular processes ranging from DNA repair to immune surveillance. Nevertheless, many nucleases have unknown or poorly characterized activities. Elucidating nuclease substrate specificities and co-factors can support a more definitive understanding of cellular mechanisms in physiology and disease. Using fluorescence-based methods, we present a quick, safe, cost-effective, and real-time versatile nuclease assay, which uniquely studies nuclease enzyme kinetics. In conjunc
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- Sheppard, Emily C
- Rogers, Sally
- Harmer, Nicholas J
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Sheppard, E. C., Rogers, S., Harmer, N. J., & Chahwan, R. (2019). A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity. Scientific Reports, 9(1), 8853. https://doi.org/10.1038/s41598-019-45356-z
