Superresolution imaging of amyloid fibrils with binding-activated probes

Ries, Jonas; Udayar, Vinod; Soragni, Alice; Hornemann, Simone; Nilsson, K Peter R; Riek, Roland; Hock, Christoph; Ewers, Helge; Aguzzi, Adriano A; Rajendran, Lawrence

doi:10.1021/cn400091m

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Ries, J., Udayar, V., Soragni, A., Hornemann, S., Nilsson, K. P. R., Riek, R., Hock, C., Ewers, H., Aguzzi, A. A., & Rajendran, L. (2013). Superresolution imaging of amyloid fibrils with binding-activated probes. ACS Chemical Neuroscience, 4(7), 1057–1061. https://doi.org/10.1021/cn400091m

Abstract

Protein misfolding into amyloid-like aggregates underlies many neurodegenerative diseases. Thus, insights into the structure and function of these amyloids will provide valuable information on the pathological mechanisms involved and aid in the design of improved drugs for treating amyloid-based disorders. However, determining the structure of endogenous amyloids at high resolution has been difficult. Here we employ binding-activated localization microscopy (BALM) to acquire superresolution images of α-synuclein amyloid fibrils with u