B cell clonal expansion and mutation in the immunoglobulin heavy chain variable domain in response to Pfs230 and Pfs25 malaria vaccines

Coelho, Camila H; Galson, Jacob D; Trück, Johannes; Duffy, Patrick E

doi:10.1016/j.ijpara.2021.11.008

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Coelho, C. H., Galson, J. D., Trück, J., & Duffy, P. E. (2022). B cell clonal expansion and mutation in the immunoglobulin heavy chain variable domain in response to Pfs230 and Pfs25 malaria vaccines. International Journal for Parasitology, 52(11), 707–710. https://doi.org/10.1016/j.ijpara.2021.11.008

Abstract

Malaria transmission-blocking vaccines induce antibodies that target Plasmodium in the mosquito vector. We recently reported that Pfs230 vaccine achieves activity superior to Pfs25 in humans. Here, we describe clonal expansion in the variable region of immunoglobulin heavy chains (VH) of antigen-specific single B cells collected from humans immunised with Pfs230D1-EPA or Pfs25-EPA conjugate vaccines formulated in Alhydrogel®. Based on studies of CD27+ memory B cells following Pfs230 vaccination, clonal expansion and somatic hypermutat