The influence of centrifugation and incubation temperatures on various veterinary and human chlamydial species

Onorini, Delia; Donati, Manuela; Marti, Hanna; Biondi, Roberta; Levi, Aurora; Nufer, Lisbeth; Prähauser, Barbara; Rigamonti, Sara; Vicari, Nadia; Borel, Nicole

doi:10.1016/j.vetmic.2019.04.012

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Onorini, D., Donati, M., Marti, H., Biondi, R., Levi, A., Nufer, L., Prähauser, B., Rigamonti, S., Vicari, N., & Borel, N. (2019). The influence of centrifugation and incubation temperatures on various veterinary and human chlamydial species. Veterinary Microbiology, 233, 11–20. https://doi.org/10.1016/j.vetmic.2019.04.012

Abstract

The Chlamydiaceae are Gram-negative bacteria causing diseases in humans and in both, endothermic (mammals and birds) and poikilothermic (e.g. reptiles, amphibians) animals. As most chlamydial species described today were isolated from humans and endothermic animals, the commonly used culturing temperature in vitro is 37 °C, although the centrifugation temperature during experimental infection, a technique necessary to improve the infection rate, may vary from 25 to 37 °C. The aim of this study was to investigate the influence of diffe