Publication: Comparing the performance of 12S mitochondrial primers for fish environmental DNA across ecosystems
Comparing the performance of 12S mitochondrial primers for fish environmental DNA across ecosystems
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Polanco F, A., Richards, E., Flück, B., Valentini, A., Altermatt, F., Brosse, S., Walser, J., Eme, D., Marques, V., Manel, S., Albouy, C., Dejean, T., & Pellissier, L. (2021). Comparing the performance of 12S mitochondrial primers for fish environmental DNA across ecosystems. Environmental DNA, 3, 1113–1127. https://doi.org/10.1002/edn3.232
Abstract
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Abstract
Through the development of environmental DNA (eDNA) metabarcoding, in situ monitoring of organisms is becoming easier and promises a revolution in our approaches to detect changes in biodiversity over space and time. A cornerstone of eDNA approach is the development of primer pairs that allow amplifying the DNA of specific taxonomic groups, which is then used to link the DNA sequence to taxonomic identification. Here, we propose a framework for comparing primer pairs regarding (a) their capacity to bind and amplify a broad coverage of
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- Polanco F, Andrea
- Richards, Eilísh
- Flück, Benjamin
- Valentini, Alice
- Altermatt, Florian
- Brosse, Sébastien
- Walser, Jean‐Claude
- Eme, David
- Marques, Virginie
- Manel, Stéphanie
- Albouy, Camille
- Dejean, Tony
- Pellissier, Loïc
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Polanco F, A., Richards, E., Flück, B., Valentini, A., Altermatt, F., Brosse, S., Walser, J., Eme, D., Marques, V., Manel, S., Albouy, C., Dejean, T., & Pellissier, L. (2021). Comparing the performance of 12S mitochondrial primers for fish environmental DNA across ecosystems. Environmental DNA, 3, 1113–1127. https://doi.org/10.1002/edn3.232
