Publication: Next-generation RNA sequencing of archival formalin-fixed paraffin-embedded urothelial bladder cancer
Next-generation RNA sequencing of archival formalin-fixed paraffin-embedded urothelial bladder cancer
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Liu, Y., Noon, A. P., Aguiar Cabeza, E., Shen, J., Kuk, C., Ilczynski, C., Ni, R., Sukhu, B., Chan, K., Barbosa-Morais, N. L., Hermanns, T., Blencowe, B. J., Azad, A., van der Kwast, T. H., Catto, J. W. F., Zlotta, A. R., & Wrana, J. L. (2014). Next-generation RNA sequencing of archival formalin-fixed paraffin-embedded urothelial bladder cancer. European Urology, 66, 982–986. https://doi.org/10.1016/j.eururo.2014.07.045
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Molecular profiling of individual cancers is key to personalised medicine. While sequencing technologies have required stringent sample collection and handling, recent technical advances offer sequencing from tissues collected in routine practice and tissues already stored in archives. In this paper, we establish methods for whole-transcriptome RNA sequencing (RNA-seq) from formalin-fixed paraffin-embedded tissues. We obtain average RNA-seq reads of >100 million per sample using the Illumina HiSeq2000 platform. We find high concordanc
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Liu, Y., Noon, A. P., Aguiar Cabeza, E., Shen, J., Kuk, C., Ilczynski, C., Ni, R., Sukhu, B., Chan, K., Barbosa-Morais, N. L., Hermanns, T., Blencowe, B. J., Azad, A., van der Kwast, T. H., Catto, J. W. F., Zlotta, A. R., & Wrana, J. L. (2014). Next-generation RNA sequencing of archival formalin-fixed paraffin-embedded urothelial bladder cancer. European Urology, 66, 982–986. https://doi.org/10.1016/j.eururo.2014.07.045