Publication: In situ mapping of innate lymphoid cells in human skin: evidence for remarkable differences between normal and inflamed skin
In situ mapping of innate lymphoid cells in human skin: evidence for remarkable differences between normal and inflamed skin
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Brüggen, M.-C., Bauer, W. M., Reininger, B., Clim, E., Captarencu, C., Steiner, G. E., Brunner, P. M., Meier, B., French, L. E., & Stingl, G. (2016). In situ mapping of innate lymphoid cells in human skin: evidence for remarkable differences between normal and inflamed skin. Journal of Investigative Dermatology, 136(12), 2396–2405. https://doi.org/10.1016/j.jid.2016.07.017
Abstract
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Abstract
Although innate lymphoid cells (ILCs) have recently been identified also in skin, their role in this organ remains poorly understood. In this study, we aimed at developing a technique to assess ILCs in situ and to determine their topographical distribution in human skin. We collected lesional skin biopsies from patients with atopic dermatitis and psoriasis (both n = 13) and normal human skin from healthy controls. After establishing immunofluorescence ILC in situ stainings, we developed an analysis approach (gating combined with manua
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- Brüggen, Marie-Charlotte
- Bauer, Wolfgang M
- Reininger, Bärbel
- Steiner, Georg E
- Brunner, Patrick M
- Meier, Barbara
- French, Lars E
- Stingl, Georg
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Brüggen, M.-C., Bauer, W. M., Reininger, B., Clim, E., Captarencu, C., Steiner, G. E., Brunner, P. M., Meier, B., French, L. E., & Stingl, G. (2016). In situ mapping of innate lymphoid cells in human skin: evidence for remarkable differences between normal and inflamed skin. Journal of Investigative Dermatology, 136(12), 2396–2405. https://doi.org/10.1016/j.jid.2016.07.017
