Human stem cell-based three-dimensional microtissues for advanced cardiac cell therapies

Emmert, Maximilian Y; Wolint, Petra; Wickboldt, Nadine; Gemayel, Gino; Weber, Benedikt; Brokopp, Chad E; Boni, Alessandro; Falk, Volkmar; Bosman, Alexis; Jaconi, Marisa E; Hoerstrup, Simon P

doi:10.5167/uzh-90509

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Emmert, M. Y., Wolint, P., Wickboldt, N., Gemayel, G., Weber, B., Brokopp, C. E., Boni, A., Falk, V., Bosman, A., Jaconi, M. E., & Hoerstrup, S. P. (2013). Human stem cell-based three-dimensional microtissues for advanced cardiac cell therapies. Biomaterials, 34(27), 6339–6354. https://doi.org/10.1016/j.biomaterials.2013.04.034

Abstract

Cardiac stem cell therapy has been proposed as a therapy option to treat the diseased myocardium. However, the low retention rate of transplanted single-cell suspensions remains a major issue of current therapy strategies. Therefore, the concept of scaffold-free cellular self-assembly into three-dimensional microtissues (3D-MTs) prior to transplantation may be beneficial to enhance retention and survival. We compared clinically relevant, human stem cell sources for their ability to generate 3D-MTs with particular regards to formation