Soste, Martin; Hrabakova, Rita; Wanka, Stefanie; Melnik, Andre; Boersema, Paul; Maiolica, Alessio; Wernas, Timon; Tognetti, Marco; von Mering, Christian; Picotti, Paola (2014). A sentinel protein assay for simultaneously quantifying cellular processes. Nature Methods, 11(10):1045-1048.
We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.
We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.
| Item Type: | Journal Article, refereed, original work |
|---|---|
| Communities & Collections: | 07 Faculty of Science > Institute of Molecular Life Sciences
08 Research Priority Programs > Evolution in Action: From Genomes to Ecosystems |
| Dewey Decimal Classification: | 570 Life sciences; biology |
| Scopus Subject Areas: | Life Sciences > Biotechnology
Life Sciences > Biochemistry Life Sciences > Molecular Biology Life Sciences > Cell Biology |
| Date: | October 2014 |
| Deposited On: | 17 Nov 2014 16:01 |
| Last Modified: | 30 Jul 2020 15:07 |
| Publisher: | Nature Publishing Group |
| ISSN: | 1548-7091 |
| Funders: | SystemX.ch |
| OA Status: | Closed |
| Publisher DOI: | https://doi.org/10.1038/nmeth.3101 |
| PubMed ID: | 25194849 |
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Soste, Martin