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Early expression of glutamate transporter proteins in ramified microglia after controlled cortical impact injury in the rat

van Landeghem, F K; Stover, J F; Bechmann, I; Brück, W; Unterberg, A; Bührer, C; von Deimling, A (2001). Early expression of glutamate transporter proteins in ramified microglia after controlled cortical impact injury in the rat. Glia, 35(3):167-179.

Abstract

Traumatic brain injury is followed by increased extracellular glutamate concentration. Uptake of glutamate is mainly mediated by the glial glutamate transporters GLAST and GLT-1. Extent and distribution of GLAST and GLT-1 were studied in a rat model of controlled cortical impact injury (CCII). Western Blot analysis revealed lowest levels of GLAST and GLT-1 with a decrease by 40%-54% and 42%-49% between 24 and 72 h posttrauma. By 8 h after CCII, CSF glutamate levels were increased (10.5 microM vs. 2.56 microM in controls; P < 0.001), reaching maximum values by 48 h. A significant increase in de novo GLAST and GLT-1 expressing ramified microglia was observed within 4 h, reached a stable level by 48 h, and remained high up to 72 h after CCII. Furthermore, ramified microglia de novo expressed the neuronal glutamate transporter EAAC1 after CCII. Following CCII, GLAST/GLT-1 and GFAP coexpressing astrocytes were immediately reduced, reaching minimum levels within 8 h. This reduction of expression could be either due to protein downregulation or loss of astrocytes. At 72 h, a marked population of GLAST- and GLT-1-positive reactive astrocytes appeared. These results support the hypothesis that reduced astrocytic GLAST and GLT-1 protein levels following CCII contribute to evolving secondary injury. Microglia are capable of de novo expressing glutamate transporter proteins, indicating that the expression of glial and neuronal glutamate transporters is not restricted to a specific glial or neuronal lineage. Ramified microglia may play an important compensatory role in the early regulation of extracellular glutamate after CCII.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Intensive Care Medicine
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Life Sciences > Neurology
Life Sciences > Cellular and Molecular Neuroscience
Uncontrolled Keywords:Cellular and Molecular Neuroscience, Neurology
Language:English
Date:September 2001
Deposited On:18 Sep 2009 09:17
Last Modified:02 Nov 2024 02:36
Publisher:Wiley-Blackwell
ISSN:0894-1491
OA Status:Closed
Publisher DOI:https://doi.org/10.1002/glia.1082
PubMed ID:11494408

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