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Destruction of a distal hypoxia response element abolishes trans-activation of the PAG1 gene mediated by HIF-independent chromatin looping

Schörg, Alexandra Sabine; Santambrogio, Sara; Platt, James L; Schödel, Johannes; Lindenmeyer, Maja T; Cohen, Clemens D; Schrödter, Katrin; Mole, David R; Wenger, Roland H; Hoogewijs, David (2015). Destruction of a distal hypoxia response element abolishes trans-activation of the PAG1 gene mediated by HIF-independent chromatin looping. Nucleic Acids Research:1-15.

Abstract

A crucial step in the cellular adaptation to oxygen deficiency is the binding of hypoxia-inducible factors (HIFs) to hypoxia response elements (HREs) of oxygen-regulated genes. Genome-wide HIF- 1alpha/2alpha/Beta DNA-binding studies revealed that the majority of HREs reside distant to the promoter regions, but the function of these distal HREs has only been marginally studied in the genomic context. We used chromatin immunoprecipitation (ChIP), gene editing (TALEN) and chromosome conformation capture (3C) to localize and functionally characterize a 82 kb upstream HRE that solely drives oxygen-regulated expression of the newly identified HIF target gene PAG1. PAG1, a transmembrane adaptor protein involved in Src signalling, was hypoxically induced in various cell lines andmouse tissues. ChIP and reporter gene assays demonstrated that the −82 kb HRE regulates PAG1, but not an equally distant gene further upstream, by direct interaction with HIF. Ablation of the consensus HRE motif abolished the hypoxic induction of PAG1 but not general oxygen signalling. 3C assays revealed that the −82 kb HRE physically associates with thePAG1 promoter region, independent of HIF-DNA interaction. These results demonstrate a constitutive interaction between the −82 kb HRE and the PAG1 promoter, suggesting a physiologically important rapid response to hypoxia.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology

04 Faculty of Medicine > Zurich Center for Integrative Human Physiology (ZIHP)
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > Genetics
Language:English
Date:2015
Deposited On:27 May 2015 12:34
Last Modified:13 Oct 2024 01:38
Publisher:Oxford University Press
ISSN:0305-1048
Funders:Forschungskredit, University of Zürich, Sassella Stiftung, Zürich, Flemish FWO, postdoctoral fellowship, European Commission, Marie Curie IEF Postdoctoral Fellowship, State Secretariat of Education, Research and Innovation, Bern, Switzerland, KFSP Tumor Oxygenation of the University of Z¨urich, Swiss National Science Foundation (31003A 146203), Wellcome Trust (088182/Z/09/Z), Biobank ERCBKFB (Else Kröner-Fresenius-Foundation), Funding for open access charge: University of Zurich
OA Status:Gold
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/nar/gkv506
Project Information:
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  • Project Title: Forschungskredit, University of Zürich
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  • Project Title: Sassella Stiftung, Zürich
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  • Project Title: Flemish FWO, postdoctoral fellowship
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  • Project Title: European Commission, Marie Curie IEF Postdoctoral Fellowship
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  • Project Title: State Secretariat of Education, Research and Innovation, Bern, Switzerland
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  • Project Title: KFSP Tumor Oxygenation of the University of Z¨urich
  • Funder: SNSF
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  • Project Title: Swiss National Science Foundation (31003A 146203)
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  • Project Title: Wellcome Trust (088182/Z/09/Z)
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  • Project Title: Biobank ERCBKFB (Else Kröner-Fresenius-Foundation)
  • Funder:
  • Grant ID:
  • Project Title: Funding for open access charge: University of Zurich
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