Header

UZH-Logo

Maintenance Infos

Genotyping coronaviruses associated with feline infectious peritonitis


Lewis, C S; Porter, E; Matthews, D; Kipar, A; Tasker, S; Helps, C R; Siddell, S G (2015). Genotyping coronaviruses associated with feline infectious peritonitis. Journal of General Virology, 96(Pt 6):1358-1368.

Abstract

Feline coronavirus (FCoV) infections are endemic among cats worldwide. The majority of infections are asymptomatic or result in only mild enteric disease. However, approximately 5 % of cases develop feline infectious peritonitis (FIP), a systemic disease that is a frequent cause of death in young cats. In this study, we report the complete coding genome sequences of six FCoVs: three from faecal samples from healthy cats and three from tissue lesion samples from cats with confirmed FIP. The six samples were obtained over a period of 8 weeks at a single-site cat rescue and rehoming centre in the UK. We found amino acid differences located at 44 positions across an alignment of the six virus translatomes and, at 21 of these positions, the differences fully or partially discriminated between the genomes derived from the faecal samples and the genomes derived from the tissue lesion samples. In this study, two amino acid differences fully discriminated the two classes of genomes: these were both located in the S2 domain of the virus surface glycoprotein gene. We also identified deletions in the 3c protein ORF of genomes from two of the FIP samples. Our results support previous studies that implicate S protein mutations in the pathogenesis of FIP.

Abstract

Feline coronavirus (FCoV) infections are endemic among cats worldwide. The majority of infections are asymptomatic or result in only mild enteric disease. However, approximately 5 % of cases develop feline infectious peritonitis (FIP), a systemic disease that is a frequent cause of death in young cats. In this study, we report the complete coding genome sequences of six FCoVs: three from faecal samples from healthy cats and three from tissue lesion samples from cats with confirmed FIP. The six samples were obtained over a period of 8 weeks at a single-site cat rescue and rehoming centre in the UK. We found amino acid differences located at 44 positions across an alignment of the six virus translatomes and, at 21 of these positions, the differences fully or partially discriminated between the genomes derived from the faecal samples and the genomes derived from the tissue lesion samples. In this study, two amino acid differences fully discriminated the two classes of genomes: these were both located in the S2 domain of the virus surface glycoprotein gene. We also identified deletions in the 3c protein ORF of genomes from two of the FIP samples. Our results support previous studies that implicate S protein mutations in the pathogenesis of FIP.

Statistics

Citations

Dimensions.ai Metrics
10 citations in Web of Science®
9 citations in Scopus®
10 citations in Microsoft Academic
Google Scholar™

Altmetrics

Downloads

33 downloads since deposited on 23 Jul 2015
9 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Pathology
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:June 2015
Deposited On:23 Jul 2015 08:30
Last Modified:23 Sep 2018 05:52
Publisher:Society for General Microbiology
ISSN:0022-1317
OA Status:Hybrid
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1099/vir.0.000084
PubMed ID:25667330

Download

Download PDF  'Genotyping coronaviruses associated with feline infectious peritonitis'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 2MB
View at publisher
Download PDF  'Genotyping coronaviruses associated with feline infectious peritonitis'.
Preview
Content: Published Version
Filetype: PDF
Size: 672kB
Licence: Creative Commons: Attribution 3.0 Unported (CC BY 3.0)