INTRODUCTION We tested the hypothesis that whole body vibration (WBV) is insufficient to expand satellite cell (SC) numbers 24 h post exercise, whereas WBV in combination with blood flow restriction (BFR) is. METHODS Twenty-five young men were randomly assigned to one of three groups: WBV, BFR exercise or WBVBFR. SC numbers were determined from muscle biopsies of the vastus lateralis using immunohistochemistry. RESULTS SC quantity and frequency (+99.38%, P = 0.012 and +77.1%, P = 0.010, respectively) only increased in the WBVBFR group. Similar results were obtained for the quantity and frequency of myogenin+ myonuclei (+139.0%, P < 0.001 and +148.4%, P < 0.001, respectively). CONCLUSION We conclude that modification of WBV by superimposing BFR induced activation and differentiation of SC in young men, all of which had not been observed with WBV or BFR alone. These data suggest that WBVBFR might represent a novel viable anabolic stimulus. This article is protected by copyright. All rights reserved.