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The AMOR Arabinogalactan sugar chain induces pollen-tube competency to respond to ovular guidance


Abstract

Precise directional control of pollen-tube growth by pistil tissue is critical for successful fertilization of flowering plants [ 1–3 ]. Ovular attractant peptides, which are secreted from two synergid cells on the side of the egg cell, have been identified [ 4–6 ]. Emerging evidence suggests that the ovular directional cue is not sufficient for successful guidance but that competency control by the pistil is critical for the response of pollen tubes to the attraction signal [ 1, 3, 7 ]. However, the female molecule for this competency induction has not been reported. Here we report that ovular methyl-glucuronosyl arabinogalactan (AMOR) induces competency of the pollen tube to respond to ovular attractant LURE peptides in Torenia fournieri. We developed a method for assaying the response capability of a pollen tube by micromanipulating an ovule. Using this method, we showed that pollen tubes growing through a cut style acquired a response capability in the medium by receiving a sufficient amount of a factor derived from mature ovules of Torenia. This factor, named AMOR, was identified as an arabinogalactan polysaccharide, the terminal 4-O-methyl-glucuronosyl residue of which was necessary for its activity. Moreover, a chemically synthesized disaccharide, the β isomer of methyl-glucuronosyl galactose (4-Me-GlcA-β-(1→6)-Gal), showed AMOR activity. No specific sugar-chain structure of plant extracellular matrix has been identified as a bioactive molecule involved in intercellular communication. We suggest that the AMOR sugar chain in the ovary renders the pollen tube competent to the chemotropic response prior to final guidance by LURE peptides.

Abstract

Precise directional control of pollen-tube growth by pistil tissue is critical for successful fertilization of flowering plants [ 1–3 ]. Ovular attractant peptides, which are secreted from two synergid cells on the side of the egg cell, have been identified [ 4–6 ]. Emerging evidence suggests that the ovular directional cue is not sufficient for successful guidance but that competency control by the pistil is critical for the response of pollen tubes to the attraction signal [ 1, 3, 7 ]. However, the female molecule for this competency induction has not been reported. Here we report that ovular methyl-glucuronosyl arabinogalactan (AMOR) induces competency of the pollen tube to respond to ovular attractant LURE peptides in Torenia fournieri. We developed a method for assaying the response capability of a pollen tube by micromanipulating an ovule. Using this method, we showed that pollen tubes growing through a cut style acquired a response capability in the medium by receiving a sufficient amount of a factor derived from mature ovules of Torenia. This factor, named AMOR, was identified as an arabinogalactan polysaccharide, the terminal 4-O-methyl-glucuronosyl residue of which was necessary for its activity. Moreover, a chemically synthesized disaccharide, the β isomer of methyl-glucuronosyl galactose (4-Me-GlcA-β-(1→6)-Gal), showed AMOR activity. No specific sugar-chain structure of plant extracellular matrix has been identified as a bioactive molecule involved in intercellular communication. We suggest that the AMOR sugar chain in the ovary renders the pollen tube competent to the chemotropic response prior to final guidance by LURE peptides.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
Uncontrolled Keywords:pollen-tube guidance, competency control, ovule, arabinogalactan, LURE attractant peptides, Torenia
Language:English
Date:25 April 2016
Deposited On:15 Apr 2016 17:33
Last Modified:19 Aug 2018 03:05
Publisher:Cell Press (Elsevier)
ISSN:0960-9822
OA Status:Closed
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/j.cub.2016.02.040
Project Information:
  • : FunderFP7
  • : Grant ID300042
  • : Project TitleEXEL - Formation, maintenance and differentiation of the extraembryonic endoderm lineage
  • : FunderFP7
  • : Grant ID267802
  • : Project TitleANAMULTISCALE - Analysis of Multiscale Systems Driven by Functionals

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