The aim of the present study was to investigate clotting profiles, plasma biochemical, and hematological parameters in cows with different degrees of fatty liver. The degree of fatty liver was determined based on histopathology of liver biopsies. Fifty cows referred for left displacement of the abomasum and different degrees of fatty liver were investigated. A clotting profile including prothrombin time (PT), thrombin time (TT), activated partial thromboplastin time (aPTT), and fibrinogen did not reveal differences between cows with or without fatty liver. Aspartate aminotransferase (ASAT) was the only biochemical parameter being significantly different in cows with fatty liver. The activity of ASAT could not differentiate any stage of fatty liver. Our findings demonstrate that alterations in clotting profiles in dairy cows are not related to fatty liver. Biochemical parameters are not sufficient to determine the severity of fatty liver.