Navigation auf zora.uzh.ch

Search ZORA

ZORA (Zurich Open Repository and Archive)

Single cell-laden protease-sensitive microniches for long-term culture in 3D

Lienemann, Philipp S; Rossow, Torsten; Mao, Angelo S; Vallmajo-Martin, Queralt; Ehrbar, Martin; Mooney, David J (2017). Single cell-laden protease-sensitive microniches for long-term culture in 3D. Lab on a chip, 17(4):727-737.

Abstract

Single cell-laden three-dimensional (3D) microgels that can serve to mimic stem cell niches in vitro, and are therefore termed microniches, can be efficiently fabricated by droplet-based microfluidics. In this technique an aqueous polymer solution along with a highly diluted cell solution is injected into a microfluidic device to create monodisperse pre-microgel droplets that are then solidified by a polymer crosslinking reaction to obtain monodisperse single cell-laden microniches. However, problems limiting this approach studying the fate of single cells include Poisson encapsulation statistics that result in mostly empty microniches, and cells egressing from the microniches during subsequent cell culture. Here, we present a strategy to bypass Poisson encapsulation statistics in synthetic microniches by selective crosslinking of only cell-laden pre-microgel droplets. Furthermore, we show that we can position cells in the center of the microniches, and that even in protease-sensitive microniches this greatly reduces cell egress. Collectively, we present the development of a versatile protocol that allows for unprecedented efficiency in creation of synthetic protease-sensitive microniches for probing single stem cell fate in 3D.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Obstetrics
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Physical Sciences > Bioengineering
Life Sciences > Biochemistry
Physical Sciences > General Chemistry
Physical Sciences > Biomedical Engineering
Language:English
Date:14 February 2017
Deposited On:17 May 2017 07:05
Last Modified:17 Oct 2024 01:36
Publisher:Royal Society of Chemistry
ISSN:1473-0189
OA Status:Closed
Publisher DOI:https://doi.org/10.1039/c6lc01444e
PubMed ID:28154867
Project Information:
  • Funder: SNSF
  • Grant ID: P2ELP3_161850
  • Project Title: Role of endothelial cells on controlling perivascular stem cells during tissue injury

Metadata Export

Statistics

Citations

Dimensions.ai Metrics
43 citations in Web of Science®
47 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 17 May 2017
0 downloads since 12 months
Detailed statistics

Authors, Affiliations, Collaborations

Similar Publications