Header

UZH-Logo

Maintenance Infos

Covalently circularized nanodiscs for studying membrane proteins and viral entry


Nasr, Mahmoud L; Baptista, Diego; Strauss, Mike; Sun, Zhen-Yu J; Grigoriu, Simina; Huser, Sonja; Plückthun, Andreas; Hagn, Franz; Walz, Thomas; Hogle, James M; Wagner, Gerhard (2017). Covalently circularized nanodiscs for studying membrane proteins and viral entry. Nature Methods, 14(1):49-52.

Abstract

We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter sizes and tunable shapes. Reconstitution into cNDs enhanced the quality of nuclear magnetic resonance spectra for both VDAC-1, a β-barrel membrane protein, and the G-protein-coupled receptor NTR1, an α-helical membrane protein. In addition, we used cNDs to visualize how simple, nonenveloped viruses translocate their genomes across membranes to initiate infection.

Abstract

We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter sizes and tunable shapes. Reconstitution into cNDs enhanced the quality of nuclear magnetic resonance spectra for both VDAC-1, a β-barrel membrane protein, and the G-protein-coupled receptor NTR1, an α-helical membrane protein. In addition, we used cNDs to visualize how simple, nonenveloped viruses translocate their genomes across membranes to initiate infection.

Statistics

Citations

Dimensions.ai Metrics
119 citations in Web of Science®
117 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

0 downloads since deposited on 23 Jun 2017
0 downloads since 12 months

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > Biotechnology
Life Sciences > Biochemistry
Life Sciences > Molecular Biology
Life Sciences > Cell Biology
Language:English
Date:January 2017
Deposited On:23 Jun 2017 09:43
Last Modified:28 Jul 2020 11:02
Publisher:Nature Publishing Group
ISSN:1548-7091
OA Status:Closed
Publisher DOI:https://doi.org/10.1038/nmeth.4079
PubMed ID:27869813

Download

Closed Access: Download allowed only for UZH members