Navigation auf zora.uzh.ch

Search ZORA

ZORA (Zurich Open Repository and Archive)

Consistent detection of bovine papillomavirus in lesions, intact skin and peripheral blood mononuclear cells of horses affected by hoof canker

Brandt, Simone; Schoster, Angelika; Tober, R; Kainzbauer, C; Burgstaller, J P; Haralambus, R; Steinborn, R; Hinterhofer, C; Stanek, C (2011). Consistent detection of bovine papillomavirus in lesions, intact skin and peripheral blood mononuclear cells of horses affected by hoof canker. Equine Veterinary Journal, 43(2):202-209.

Abstract

REASONS FOR PERFORMING THE STUDY: Equine hoof canker is a chronic proliferative pododermatitis of as yet unknown aetiology. Like equine sarcoid disease, canker is a therapy-resistant disorder characterised by hyperkeratosis, acanthosis and a marked tendency to recur.
HYPOTHESIS: There is an association of sarcoid-inducing bovine papillomaviruses of types 1 and 2 (BPV-1, BPV-2) with hoof canker disease.
METHODS: Using PCR-based techniques, we assessed canker tissue, intact skin and/or peripheral blood mononuclear cells (PBMCs) of 25 canker-affected horses for the presence of sarcoid-associated BPV-1 and -2.
RESULTS: Conventional PCR revealed BPV-1/-2 DNA in 24/24 canker, 12/13 skin and 10/11 PBMC DNA isolates. Using inverse PCR, full-length BPV episomes were detected in 1/5 canker specimens. Sequencing of viral early and late genes amplified from canker, intact skin and PBMC DNA of 2 cases revealed an overall identity of 98% to BPV-1. Viral DNA loads amounted to ≤16 copies per cell in canker tissue and intact skin, and to ≤0.35 copies per PBMC, as determined by quantitative PCR. Using RT-PCR, the viral major oncogene E5 was shown to be transcribed in 2/4 canker tissue specimens and 5/7 PBMC isolates. Immunocapture PCR from 7 canker and 6 skin extract supernatants revealed capsomere-associated viral DNA in one canker and one skin sample. Hoof tissue, skin and PBMCs collected from 13 individuals with no signs of canker or BPV-related malignancies scored negative throughout the experiments.
CONCLUSION: These findings suggest that the observed presence of BPV-1/-2 in canker-affected horses is not coincidental but indicative of an active contribution to hoof canker disease.
POTENTIAL RELEVANCE: The use of antivirals and/or immune modulators may help improving canker therapy.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Equine Department
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Scopus Subject Areas:Health Sciences > Equine
Language:English
Date:2011
Deposited On:29 Aug 2017 16:23
Last Modified:17 Oct 2024 01:37
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:0425-1644
OA Status:Closed
Publisher DOI:https://doi.org/10.1111/j.2042-3306.2010.00147.x
PubMed ID:21592216

Metadata Export

Statistics

Citations

Dimensions.ai Metrics
27 citations in Web of Science®
31 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

0 downloads since deposited on 29 Aug 2017
0 downloads since 12 months

Authors, Affiliations, Collaborations

Similar Publications