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Identification of Fungal T Cell Epitopes by Mass Spectrometry-Based Proteomics


Roschitzki, Bernd; LeibundGut-Landmann, Salomé (2017). Identification of Fungal T Cell Epitopes by Mass Spectrometry-Based Proteomics. In: Kalkum, Markus; Semis, Margarita. Vaccines for Invasive Fungal Infections. New York: Springer, 65-74.

Abstract

CD4(+) T cells play a key role in host defense against many fungal infections. T cells are also implicated in vaccine immunity to fungi. To date, only a small number of fungal antigens have been identified. Knowing the antigenic determinants of fungi-specific T cells greatly facilitates the detection, enumeration and characterizes the antifungal T cells and it constitutes an important step toward the design and development of vaccination strategies. This chapter describes a method of MHC-II ligand elution and mass spectrometric analysis to identify naturally processed and presented fungal peptide epitopes.

Abstract

CD4(+) T cells play a key role in host defense against many fungal infections. T cells are also implicated in vaccine immunity to fungi. To date, only a small number of fungal antigens have been identified. Knowing the antigenic determinants of fungi-specific T cells greatly facilitates the detection, enumeration and characterizes the antifungal T cells and it constitutes an important step toward the design and development of vaccination strategies. This chapter describes a method of MHC-II ligand elution and mass spectrometric analysis to identify naturally processed and presented fungal peptide epitopes.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Virology
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Molecular Biology
Life Sciences > Genetics
Uncontrolled Keywords:Fungal antigens, MHC-II ligand elution, Mass spectrometry, Naturally processed and presented T cell epitopes, T helper cells
Language:English
Date:2017
Deposited On:10 Nov 2017 08:34
Last Modified:28 Jul 2020 11:47
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:1625
ISSN:1064-3745
ISBN:978-1-4939-7103-9
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-4939-7104-6
PubMed ID:28584983

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