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Cerium chloride application promotes wound healing and cell proliferation in human foreskin fibroblasts


Ramenzoni, Liza L; Weber, Franz E; Attin, Thomas; Schmidlin, Patrick R (2017). Cerium chloride application promotes wound healing and cell proliferation in human foreskin fibroblasts. Materials, 10(6):573.

Abstract

This study investigated the effect of cerium chloride (CeCl₃) on cell migration and gene expression of human foreskin fibroblasts (HFF). HFF were exposed to three different CeCl₃ solutions (1%, 5% and 10%, w/v %) for three different time durations (1, 5 and 10 min). 72 h after exposure to CeCl₃, cell viability was assessed by MTT test. A scratch-wounded assay determined the cell migration and the width of the wound, measured at 24 h. Gene expression patterns for cyclins B1, D1 and E1 were analyzed by RT-PCR (p < 0.05, t-test). The viability proliferation increased at 1- and 5-min exposures for all CeCl₃ concentrations, in contrast to no treatment (p < 0.05 at 24 h). No influence of CeCl₃ was found after 10 min. The scratch assay showed increased cell migration up to 60% at 1 and 5 min after 24 h at 5% and 10%. Cyclin B1, D1 and E1 all showed upregulation, confirming an increase in cell proliferation. This study demonstrates that exposure time and concentration of CeCl₃ may have a positive effect on fibroblast viability and migration. Application of CeCl₃ may be beneficial as a cell-stimulating agent leading to therapeutic tissue fibrosis or more resistant tissue around teeth, when warranted, during different periodontal therapies.

Abstract

This study investigated the effect of cerium chloride (CeCl₃) on cell migration and gene expression of human foreskin fibroblasts (HFF). HFF were exposed to three different CeCl₃ solutions (1%, 5% and 10%, w/v %) for three different time durations (1, 5 and 10 min). 72 h after exposure to CeCl₃, cell viability was assessed by MTT test. A scratch-wounded assay determined the cell migration and the width of the wound, measured at 24 h. Gene expression patterns for cyclins B1, D1 and E1 were analyzed by RT-PCR (p < 0.05, t-test). The viability proliferation increased at 1- and 5-min exposures for all CeCl₃ concentrations, in contrast to no treatment (p < 0.05 at 24 h). No influence of CeCl₃ was found after 10 min. The scratch assay showed increased cell migration up to 60% at 1 and 5 min after 24 h at 5% and 10%. Cyclin B1, D1 and E1 all showed upregulation, confirming an increase in cell proliferation. This study demonstrates that exposure time and concentration of CeCl₃ may have a positive effect on fibroblast viability and migration. Application of CeCl₃ may be beneficial as a cell-stimulating agent leading to therapeutic tissue fibrosis or more resistant tissue around teeth, when warranted, during different periodontal therapies.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic of Conservative and Preventive Dentistry
04 Faculty of Medicine > Center for Dental Medicine > Clinic of Cranio-Maxillofacial Surgery
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Physical Sciences > General Materials Science
Language:English
Date:24 May 2017
Deposited On:22 Nov 2017 11:36
Last Modified:08 Apr 2020 22:01
Publisher:MDPI Publishing
ISSN:1996-1944
OA Status:Gold
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.3390/ma10060573
PubMed ID:28772932

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