This chapter analyzes the use of Twitter by two female Latin American presidents: Cristina Fernández de Kirchner (Argentina) and Dilma Rousseff (Brazil). The analysis proceeds along the following two lines of inquiry. First, the authors examine whether these presidents use Twitter to broadcast information in a traditional way or deploy the interactive features provided by the tool to engage in a “digital dialogue” with their followers. Second, they use content analysis to identify for which purposes these presidents use Twitter (i.e. the functions and style of their tweets) and to what extent they tweet about gender-related topics (i.e. gender policy advocacy). The study is based on qualitative content data from two different time periods (during and beyond electoral campaigns).