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The Role of B Cells in Carriage and Clearance of Mycoplasma pneumoniae From the Respiratory Tract of Mice


Abstract

BACKGROUND: Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown.
METHODS: In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans.
RESULTS: Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, whereas this enabled μMT mice to clear pulmonary Mp infection.
CONCLUSIONS: We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.

Abstract

BACKGROUND: Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown.
METHODS: In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans.
RESULTS: Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, whereas this enabled μMT mice to clear pulmonary Mp infection.
CONCLUSIONS: We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.

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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Children's Hospital Zurich > Medical Clinic
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:4 January 2018
Deposited On:16 Jan 2018 07:26
Last Modified:20 Feb 2018 09:00
Publisher:Oxford University Press
ISSN:0022-1899
OA Status:Closed
Publisher DOI:https://doi.org/10.1093/infdis/jix559
PubMed ID:29099932

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