Immunohistochemical expression of collagen types I, III, IV and α-actin in the uterine horns of nulliparous and multiparous beagles

Abstract

Collagen and smooth muscle cells play essential roles in the remodelling of uterine tissue during pregnancy and involution. To investigate the immunoreactivity and distribution pattern of collagen types I, III, IV and smooth muscle α-actin resulting from these processes, two homogenous groups of nulliparous and multiparous beagles were evaluated by immunohistochemistry. Immunostaining patterns of collagens I and III delineated the uterine connective tissue fibers and revealed their dual presence within fibers of both beagle groups. Collagen III staining, in particular, was more pronounced and especially evident in superficial fiber sections. The numerous, large arteries in the myometrial stratum vasculare of multiparous uteri exhibited a highly thickened intima, which distinctly expressed type I and III collagens. Intense collagen IV immunolabeling was discernable in the basement membranes of vascular endothelia and smooth muscle cells. Staining of the basement membranes of the luminal and glandular epithelia, conversely, was either absent or very weak. No difference in the immunoreactivity and distribution of the assessed collagens and actin could be detected between nulliparous and multiparous dogs. Overall, and with the exception of sclerotic arteries, immunohistochemical analysis revealed that the expression of uterine collagens and actin does not change in the uterus of multiparous beagles, even after seven elapsed pregnancies.