Abstract
Two-photon microscopy is able to produce exquisite and informative functional images of cells and blood vessels in living animals or tissue,1 especially when combined with genetically-encoded indicators of cellular activity.2 Since these imaging techniques typically produce too much data to analyse manually, a number of automated or semi-automated image analysis approaches have been developed. However, even aside from the algorithms themselves, many factors can complicate analysis workflows.
Barrett, Matthew J P