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Isolation and detection of the chlorophyll catabolite hydroxylating activity from capsicum annuum chromoplasts


Hauenstein, Mareike; Hörtensteiner, Stefan (2017). Isolation and detection of the chlorophyll catabolite hydroxylating activity from capsicum annuum chromoplasts. Bio-protocol, 7(18):e2561.

Abstract

Hydroxylation of chlorophyll catabolites at the so-called C32 position (Hauenstein et al., 2016) is commonly found in all plant species analyzed to date. Here we describe an in vitro hydroxylation assay using Capsicum annuum chromoplast membranes as a source of the hydroxylating activity, which converts the substrate epi-pFCC (epi-primary Fluorescent Chlorophyll Catabolite) (Mühlecker et al., 2000) to epi-pFCC-OH.

Abstract

Hydroxylation of chlorophyll catabolites at the so-called C32 position (Hauenstein et al., 2016) is commonly found in all plant species analyzed to date. Here we describe an in vitro hydroxylation assay using Capsicum annuum chromoplast membranes as a source of the hydroxylating activity, which converts the substrate epi-pFCC (epi-primary Fluorescent Chlorophyll Catabolite) (Mühlecker et al., 2000) to epi-pFCC-OH.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Language:English
Date:2017
Deposited On:26 Feb 2018 16:14
Last Modified:16 Mar 2018 05:38
Publisher:Bio-protocol
ISSN:2331-8325
OA Status:Green
Publisher DOI:https://doi.org/10.21769/BioProtoc.2561

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