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Generation of spheres from dental epithelial stem cells


Natsiou, Despoina; Granchi, Zoraide; Mitsiadis, Thimios A; Jimenez-Rojo, Lucia (2017). Generation of spheres from dental epithelial stem cells. Frontiers in Physiology, 8:7.

Abstract

Thethree-dimensional sphere model has already been established as an important tool in fundamental sciences. This model facilitates the study of a variety of biological processes including stem cell/niche functions and tissue responses to injury and drugs. Here we describe the complete protocol for theformation of spheres originated from the epithelium of rodent incisors. In addition, we show that in these spheres cell proliferation is maintained, as well as the expression of several key molecules characterizing stem cells such as Sox2 and p63. These epithelial dentospheres could be used as anmodel system for stem cell research purposes.

Abstract

Thethree-dimensional sphere model has already been established as an important tool in fundamental sciences. This model facilitates the study of a variety of biological processes including stem cell/niche functions and tissue responses to injury and drugs. Here we describe the complete protocol for theformation of spheres originated from the epithelium of rodent incisors. In addition, we show that in these spheres cell proliferation is maintained, as well as the expression of several key molecules characterizing stem cells such as Sox2 and p63. These epithelial dentospheres could be used as anmodel system for stem cell research purposes.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Institute of Oral Biology
Dewey Decimal Classification:610 Medicine & health
Scopus Subject Areas:Life Sciences > Physiology
Health Sciences > Physiology (medical)
Language:English
Date:2017
Deposited On:27 Feb 2018 18:46
Last Modified:24 May 2024 03:35
Publisher:Frontiers Research Foundation
ISSN:1664-042X
OA Status:Gold
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.3389/fphys.2017.00007
PubMed ID:28154538
Project Information:
  • : FunderSNSF
  • : Grant ID31003A_135633
  • : Project TitleGeneration of amelogenesis imperfecta: understanding the responsible cellular and molecular mechanisms
  • Content: Published Version
  • Licence: Creative Commons: Attribution 4.0 International (CC BY 4.0)