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Antibiotic Algae by Chemical Surface Engineering


Kerschgens, Isabel P; Gademann, Karl (2018). Antibiotic Algae by Chemical Surface Engineering. Chembiochem, 19(5):439-443.

Abstract

Chemical cell-surface engineering is atool for modifying and alteringcellular functions. Herein, we report the introduction of an antibiotic phenotype to the green alga Chlamydomonas reinhardtii by chemically modifyingits cell surface. Flow cytom- etry and confocal microscopy studies demonstrated that a hybrid of the antibiotic vancomycin anda4-hydroxyproline oligomer binds reversibly to the cell wall without affecting the viability or motility of the cells. The modified cells were used to inhibit bacterial growth of Gr am-positive Bacillus subtilis cultures.Deliveryofthe antibiotic from the microalgaetothe bacterialcells was verified by microsco py.Our studies provid e compelling evidence that 1) chemical surfaceengineering con- stitutesauseful tool for the introductionofnew,previously unknown functionality,and 2) living microalgae can serve as new platforms for drug delivery.

Abstract

Chemical cell-surface engineering is atool for modifying and alteringcellular functions. Herein, we report the introduction of an antibiotic phenotype to the green alga Chlamydomonas reinhardtii by chemically modifyingits cell surface. Flow cytom- etry and confocal microscopy studies demonstrated that a hybrid of the antibiotic vancomycin anda4-hydroxyproline oligomer binds reversibly to the cell wall without affecting the viability or motility of the cells. The modified cells were used to inhibit bacterial growth of Gr am-positive Bacillus subtilis cultures.Deliveryofthe antibiotic from the microalgaetothe bacterialcells was verified by microsco py.Our studies provid e compelling evidence that 1) chemical surfaceengineering con- stitutesauseful tool for the introductionofnew,previously unknown functionality,and 2) living microalgae can serve as new platforms for drug delivery.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Scopus Subject Areas:Life Sciences > Biochemistry
Life Sciences > Molecular Medicine
Life Sciences > Molecular Biology
Physical Sciences > Organic Chemistry
Language:English
Date:2018
Deposited On:03 Apr 2018 15:50
Last Modified:29 Jul 2020 07:07
Publisher:Wiley-VCH Verlag
ISSN:1439-4227
Additional Information:For accepted manuscripts: This is the peer reviewed version of the following article: Kerschgens, Isabel P; Gademann, Karl (2018). Antibiotic Algae by Chemical Surface Engineering. Chembiochem, 19(5):439-443., which has been published in final form at https://doi.org/10.1002/cbic.201700553 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-820227.html#terms)
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1002/cbic.201700553
Project Information:
  • : FunderSNSF
  • : Grant ID200020_163151
  • : Project TitleDirecting Neurite Outgrowth through Synthetic Natural Products

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