Header

UZH-Logo

Maintenance Infos

The role of norepinephrine and alpha-adrenergic receptors in acute stress-induced changes in granulocytes and monocytes


Beis, Daniel; von Känel, Roland; Heimgartner, Nadja; Zuccarella-Hackl, Claudia; Bürkle, Alexander; Ehlert, Ulrike; Wirtz, Petra H (2018). The role of norepinephrine and alpha-adrenergic receptors in acute stress-induced changes in granulocytes and monocytes. Psychosomatic Medicine, 80(7):649-658.

Abstract

OBJECTIVE: Acute stress induces redistribution of circulating leucocytes in humans. Whereas effects on lymphocytes as adaptive immune cells are well-understood, the mechanisms underlying stress effects on granulocytes and monocytes as innate immune blood cells are still elusive. We investigated whether the stress hormone norepinephrine (NE) and α-adrenergic receptors (α-ADRs) may play a mediating role.

METHODS: In a stress study, we cross-sectionally tested in 44 healthy men for associations between stress-induced NE increases and simultaneous granulocyte and monocyte cell count increases, as measured immediately before and several times after the Trier Social Stress Test. In a subsequent infusion study, 21 healthy men participated in three different experimental trials with sequential infusions of 1 and 15-min duration with varying substances [saline as placebo, the non-specific α-ADR blocker phentolamine (2.5mg/min), and NE (5μg/min)]: trial1=saline+saline, trial2=saline+NE, trial3=phentolamine+NE. Granulocyte and monocyte cell numbers were assessed before, immediately after, 10min, and 30min after infusion procedures.

RESULTS: In the stress study, higher NE related to higher neutrophil stress changes (β=.31,p=.045, Rchange=.09), but not epinephrine stress changes. In the infusion study, saline+NE induced significant increases in neutrophil (F(3/60)=43.50,p<.001,η=.69) and monocyte (F(3/60)=18.56,p<.001,η=.48) numbers compared to saline+saline. With phentolamine+NE, neutrophil (F(3/60)=14.41,p<.001,η=.42) and monocyte counts (F(2.23/44.6)=4.32,p=.016,η=.18) remained increased compared to saline+saline but were lower compared to saline+NE (neutrophils: F(3/60)=19.55,p<.001,η=.494, monocytes: F(3/60)=2.54,p=.065,η=.11) indicating partial mediation by α-ADRs. Trials did not differ in eosinophil and basophil count reactivity.

CONCLUSIONS: Our findings suggest that NE-induced immediate increases in neutrophil and monocyte numbers resemble psychosocial stress effects and can be reduced by blockade of α-ADRs.

Abstract

OBJECTIVE: Acute stress induces redistribution of circulating leucocytes in humans. Whereas effects on lymphocytes as adaptive immune cells are well-understood, the mechanisms underlying stress effects on granulocytes and monocytes as innate immune blood cells are still elusive. We investigated whether the stress hormone norepinephrine (NE) and α-adrenergic receptors (α-ADRs) may play a mediating role.

METHODS: In a stress study, we cross-sectionally tested in 44 healthy men for associations between stress-induced NE increases and simultaneous granulocyte and monocyte cell count increases, as measured immediately before and several times after the Trier Social Stress Test. In a subsequent infusion study, 21 healthy men participated in three different experimental trials with sequential infusions of 1 and 15-min duration with varying substances [saline as placebo, the non-specific α-ADR blocker phentolamine (2.5mg/min), and NE (5μg/min)]: trial1=saline+saline, trial2=saline+NE, trial3=phentolamine+NE. Granulocyte and monocyte cell numbers were assessed before, immediately after, 10min, and 30min after infusion procedures.

RESULTS: In the stress study, higher NE related to higher neutrophil stress changes (β=.31,p=.045, Rchange=.09), but not epinephrine stress changes. In the infusion study, saline+NE induced significant increases in neutrophil (F(3/60)=43.50,p<.001,η=.69) and monocyte (F(3/60)=18.56,p<.001,η=.48) numbers compared to saline+saline. With phentolamine+NE, neutrophil (F(3/60)=14.41,p<.001,η=.42) and monocyte counts (F(2.23/44.6)=4.32,p=.016,η=.18) remained increased compared to saline+saline but were lower compared to saline+NE (neutrophils: F(3/60)=19.55,p<.001,η=.494, monocytes: F(3/60)=2.54,p=.065,η=.11) indicating partial mediation by α-ADRs. Trials did not differ in eosinophil and basophil count reactivity.

CONCLUSIONS: Our findings suggest that NE-induced immediate increases in neutrophil and monocyte numbers resemble psychosocial stress effects and can be reduced by blockade of α-ADRs.

Statistics

Citations

Dimensions.ai Metrics

Altmetrics

Downloads

13 downloads since deposited on 12 Jul 2018
13 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:06 Faculty of Arts > Institute of Psychology
Dewey Decimal Classification:150 Psychology
Uncontrolled Keywords:Applied Psychology, Psychiatry and Mental health
Language:English
Date:29 June 2018
Deposited On:12 Jul 2018 08:42
Last Modified:29 Aug 2018 01:02
Publisher:Lippincott Williams & Wilkins
ISSN:0033-3174
Additional Information:This is a non-final version of an article published in final form in "Psychosomatic Medicine".
OA Status:Green
Publisher DOI:https://doi.org/10.1097/PSY.0000000000000620
PubMed ID:29965944
Project Information:
  • : FunderSNSF
  • : Grant IDPP00P1_128565
  • : Project TitlePsychoneurobiological mechanisms in essential hypertension and coronary heart disease
  • : FunderSNSF
  • : Grant ID320030_122406
  • : Project TitleThe effects of alpha- adrenergic blockade on norepinephrine-induced changes in blood lipids, procoagulant activity, and inflammatory cytokines in essential hypertension:associations with psychological factors?
  • : FunderDFG
  • : Grant ID38/550-1
  • : Project Title

Download

Download PDF  'The role of norepinephrine and alpha-adrenergic receptors in acute stress-induced changes in granulocytes and monocytes'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 1MB
View at publisher
Publisher License