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Neurectomy preserves fast fibers when combined with tenotomy of infraspinatus muscle via upregulation of myogenesis


Flück, Martin; Valdivieso, Paola; Ruoss, Severin; von Rechenberg, Brigitte; Benn, Mario C; Meyer, Dominik C; Wieser, Karl; Gerber, Christian (2019). Neurectomy preserves fast fibers when combined with tenotomy of infraspinatus muscle via upregulation of myogenesis. Muscle & Nerve, 59(1):100-107.

Abstract

INTRODUCTION We evaluated the contribution of denervation-related molecular processes to rotator cuff muscle degeneration after tendon release. METHODS We assessed the levels of myogenic (myogenin and myogenic differentiation factor [myoD]) and pro-adipogenic (peroxisome proliferator-activated receptor gamma) transcription factors; the denervation-associated proteins tenascin-C, laminin-2, and calcium/calmodulin dependent kinase II (CaMKII); and cellular alterations in sheep after infraspinatus tenotomy (TEN), suprascapular neurectomy (NEU), or both (TEN-NEU). RESULTS Extracellular ground substance increased at the expense of contractile tissue 16 weeks after surgery, correlating with CaMKII isoform levels. Sheep undergoing NEU and TEN-NEU had exaggerated infraspinatus atrophy and increased fast fibers compared with TEN sheep. The βMCaMKII isoform levels increased with tenotomy, and myoD levels tripled after denervation and were associated with slow fibers. DISCUSSION In sheep, denervation did not affect muscle-to-fat conversion after tenotomy of the infraspinatus. Furthermore, concurrent neurectomy mitigated the loss of fast fibers after tenotomy by inducing a fast-contractile phenotype. This article is protected by copyright. All rights reserved.

Abstract

INTRODUCTION We evaluated the contribution of denervation-related molecular processes to rotator cuff muscle degeneration after tendon release. METHODS We assessed the levels of myogenic (myogenin and myogenic differentiation factor [myoD]) and pro-adipogenic (peroxisome proliferator-activated receptor gamma) transcription factors; the denervation-associated proteins tenascin-C, laminin-2, and calcium/calmodulin dependent kinase II (CaMKII); and cellular alterations in sheep after infraspinatus tenotomy (TEN), suprascapular neurectomy (NEU), or both (TEN-NEU). RESULTS Extracellular ground substance increased at the expense of contractile tissue 16 weeks after surgery, correlating with CaMKII isoform levels. Sheep undergoing NEU and TEN-NEU had exaggerated infraspinatus atrophy and increased fast fibers compared with TEN sheep. The βMCaMKII isoform levels increased with tenotomy, and myoD levels tripled after denervation and were associated with slow fibers. DISCUSSION In sheep, denervation did not affect muscle-to-fat conversion after tenotomy of the infraspinatus. Furthermore, concurrent neurectomy mitigated the loss of fast fibers after tenotomy by inducing a fast-contractile phenotype. This article is protected by copyright. All rights reserved.

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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Balgrist University Hospital, Swiss Spinal Cord Injury Center
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:1 January 2019
Deposited On:19 Sep 2018 13:18
Last Modified:24 Sep 2019 23:36
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:0148-639X
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1002/mus.26316
PubMed ID:30073680

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