Header

UZH-Logo

Maintenance Infos

The influence of the rapamycin-derivate SDZ RAD on the healing of airway anastomoses


Dutly, A E; Gaspert, A; Inci, I; Schneiter, D; Korom, S; Weder, W (2003). The influence of the rapamycin-derivate SDZ RAD on the healing of airway anastomoses. European Journal of Cardio-Thoracic Surgery, 24(1):154-158.

Abstract

Objective: Among the many immunosuppressive effects of SDZ RAD (40-0(2-hydroxyethyl)-rapamycin), a rapamycin derivative, is the inhibition of fibroblast proliferation. Since the long-term success of lung transplantation is limited by the development of bronchiolitis obliterans, a fibroblast-associated progressive luminal obstruction of the terminal bronchioli, the use of SDZ RAD as immunosuppressive in pulmonary graft recipients may counteract this process. However, reduction of fibroblast activity, posttransplant, may impair the healing of the bronchial anastomoses. Materials and methods: The cervical trachea in pigs was denuded, divided and re-anastomosed with Prolene 4-0 single stitches. Control animals (group 1, n=4) were without, and study animals (group 2, n=6) were with SDZ RAD therapy (1.25 mg/kg/day, p.o., 14 days). After 14 days, the pigs were sacrificed. The anastomoses were examined histologically, and breaking strength of tracheal strips of 5-mm width was measured. Results: All animals survived without complications. Serum levels of SDZ RAD were 30.9±8.7 ng/ml (recommended level 20-40 ng/ml). All anastomoses healed macroscopically without difference between the two groups. Breaking strength was significantly lower in the treated animals (group 1 vs. group 2: 11.75±0.35 vs. 7.69±1.39 N, P=0.01). Histology did not show a significant change in histoarchitecture between the groups. Conclusions: Although SDZ RAD significantly reduced the breaking strength of the tracheal anastomosis, no obvious histological differences between treated and untreated animals could be detected. Since this model does not reflect the clinical situation, further investigations are necessary to reveal the effect of SDZ RAD on airway wound healing in concert with a contemporary clinically used multidrug immunosuppressive regimen in allograft recipients

Abstract

Objective: Among the many immunosuppressive effects of SDZ RAD (40-0(2-hydroxyethyl)-rapamycin), a rapamycin derivative, is the inhibition of fibroblast proliferation. Since the long-term success of lung transplantation is limited by the development of bronchiolitis obliterans, a fibroblast-associated progressive luminal obstruction of the terminal bronchioli, the use of SDZ RAD as immunosuppressive in pulmonary graft recipients may counteract this process. However, reduction of fibroblast activity, posttransplant, may impair the healing of the bronchial anastomoses. Materials and methods: The cervical trachea in pigs was denuded, divided and re-anastomosed with Prolene 4-0 single stitches. Control animals (group 1, n=4) were without, and study animals (group 2, n=6) were with SDZ RAD therapy (1.25 mg/kg/day, p.o., 14 days). After 14 days, the pigs were sacrificed. The anastomoses were examined histologically, and breaking strength of tracheal strips of 5-mm width was measured. Results: All animals survived without complications. Serum levels of SDZ RAD were 30.9±8.7 ng/ml (recommended level 20-40 ng/ml). All anastomoses healed macroscopically without difference between the two groups. Breaking strength was significantly lower in the treated animals (group 1 vs. group 2: 11.75±0.35 vs. 7.69±1.39 N, P=0.01). Histology did not show a significant change in histoarchitecture between the groups. Conclusions: Although SDZ RAD significantly reduced the breaking strength of the tracheal anastomosis, no obvious histological differences between treated and untreated animals could be detected. Since this model does not reflect the clinical situation, further investigations are necessary to reveal the effect of SDZ RAD on airway wound healing in concert with a contemporary clinically used multidrug immunosuppressive regimen in allograft recipients

Statistics

Citations

Dimensions.ai Metrics
19 citations in Web of Science®
24 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

12 downloads since deposited on 05 Oct 2018
12 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:1 July 2003
Deposited On:05 Oct 2018 16:41
Last Modified:24 Nov 2018 02:53
Publisher:Oxford University Press
ISSN:1010-7940
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/s1010-7940(03)00182-9
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicenceoxford101016S1010794003001829 (Library Catalogue)
PubMed ID:12853061

Download

Download PDF  'The influence of the rapamycin-derivate SDZ RAD on the healing of airway anastomoses'.
Preview
Content: Published Version
Language: English
Filetype: PDF (Nationallizenz 142-005)
Size: 765kB
View at publisher