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Lack of IL-6 augments inflammatory response but decreases vascular permeability in bacterial meningitis


Paul, R; Koedel, U; Winkler, F; Kieseier, B C; Fontana, A; Kopf, M; Hartung, H P; Pfister, H W (2003). Lack of IL-6 augments inflammatory response but decreases vascular permeability in bacterial meningitis. Brain : a journal of neurology, 126(8):1873-1882.

Abstract

Interleukin (IL)‐6 is a multifunctional cytokine with diverse actions and has been implicated in the pathophysiology of many neurological and inflammatory disorders. In this study, we investigated the role of IL‐6 in pneumococcal meningitis. Cerebral infection in wild‐type (WT) mice caused an increase in vascular permeability and intracranial pressure (ICP), which were significantly reduced in IL‐6-/- mice. In contrast, meningitis in IL‐6-/- mice was associated with a significant increase in CSF white blood cell count compared with infected WT mice, indicating an enhanced inflammatory response. Analysis of mRNA expression in the brain showed an increase in tumour necrosis factor (TNF)‐α, IL‐1β, and macrophage inflammatory protein 2 (MIP‐2) levels, but decreased expression of granulocyte-macrophage colony‐stimulating factor in infected IL‐6-/- mice compared with infected WT controls. Similar results were obtained when rats challenged with pneumococci were systemically treated with neutralizing anti‐IL‐6 antibodies, resulting in an increased pleocytosis but at the same time a reduction of vascular permeability, brain oedema formation, and ICP, which was not accompanied by a downregulation of matrix metalloproteinases. Our data indicate that IL‐6 plays an important anti‐inflammatory role in bacterial meningitis by reducing leukocyte infiltration but contributes to the rise in intracranial pressure by increasing blood-brain barrier (BBB) permeability. These findings suggest that the migration of leukocytes across the BBB and the increase in vascular permeability are two independent processes during bacterial meningitis

Abstract

Interleukin (IL)‐6 is a multifunctional cytokine with diverse actions and has been implicated in the pathophysiology of many neurological and inflammatory disorders. In this study, we investigated the role of IL‐6 in pneumococcal meningitis. Cerebral infection in wild‐type (WT) mice caused an increase in vascular permeability and intracranial pressure (ICP), which were significantly reduced in IL‐6-/- mice. In contrast, meningitis in IL‐6-/- mice was associated with a significant increase in CSF white blood cell count compared with infected WT mice, indicating an enhanced inflammatory response. Analysis of mRNA expression in the brain showed an increase in tumour necrosis factor (TNF)‐α, IL‐1β, and macrophage inflammatory protein 2 (MIP‐2) levels, but decreased expression of granulocyte-macrophage colony‐stimulating factor in infected IL‐6-/- mice compared with infected WT controls. Similar results were obtained when rats challenged with pneumococci were systemically treated with neutralizing anti‐IL‐6 antibodies, resulting in an increased pleocytosis but at the same time a reduction of vascular permeability, brain oedema formation, and ICP, which was not accompanied by a downregulation of matrix metalloproteinases. Our data indicate that IL‐6 plays an important anti‐inflammatory role in bacterial meningitis by reducing leukocyte infiltration but contributes to the rise in intracranial pressure by increasing blood-brain barrier (BBB) permeability. These findings suggest that the migration of leukocytes across the BBB and the increase in vascular permeability are two independent processes during bacterial meningitis

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Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:4 June 2003
Deposited On:09 Oct 2018 13:54
Last Modified:24 Apr 2019 08:18
Publisher:Oxford University Press
ISSN:0006-8950
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/brain/awg171
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicenceoxford101093brainawg171 (Library Catalogue)
PubMed ID:12821529

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