Header

UZH-Logo

Maintenance Infos

Effects of μCT radiation on tissue engineered bone-like constructs


Kraehenbuehl, Thomas P; Stauber, Martin; Ehrbar, Martin; Weber, Franz; Hall, Heike; Müller, Ralph (2010). Effects of μCT radiation on tissue engineered bone-like constructs. Biomedizinische Technik. Biomedical engineering, 55(4):245-250.

Abstract

High-resolution, non-destructive imaging with micro-computed tomography (μCT) enables in situ monitoring of tissue engineered bone constructs. However, it remains controversial, if the locally imposed X-ray dose affects bone development and thus could influence the results. Here, we developed a model system for μCT monitoring of tissue engineered bone-like constructs. We examined the in vitro effects of high-resolution μCT imaging on the cellular level by using pre-osteoblastic MC3T3-E1 cells embedded into three-dimensional collagen type I matrices. We found no significantly reduced cell survival 2h after irradiation with a dose of 1.9Gy. However, 24h post-irradiation, cell survival was significantly decreased by 15% compared to non-irradiated samples. The highest dose of 7.6Gy decreased survival of the pre-osteoblastic MC3T3-E1 cells by around 40% at 2days post-irradiation. No significant increase of alkaline phosphatase (ALP) activity at 2days post-irradiation was found with a dose of 1.9Gy. However, ALP activity was significantly decreased after 7days. Using our model system, the results indicate that μCT imaging with doses as low as 1.9Gy, which is required to obtain a reasonable image quality, can induce irreparable damages on the cellular level

Abstract

High-resolution, non-destructive imaging with micro-computed tomography (μCT) enables in situ monitoring of tissue engineered bone constructs. However, it remains controversial, if the locally imposed X-ray dose affects bone development and thus could influence the results. Here, we developed a model system for μCT monitoring of tissue engineered bone-like constructs. We examined the in vitro effects of high-resolution μCT imaging on the cellular level by using pre-osteoblastic MC3T3-E1 cells embedded into three-dimensional collagen type I matrices. We found no significantly reduced cell survival 2h after irradiation with a dose of 1.9Gy. However, 24h post-irradiation, cell survival was significantly decreased by 15% compared to non-irradiated samples. The highest dose of 7.6Gy decreased survival of the pre-osteoblastic MC3T3-E1 cells by around 40% at 2days post-irradiation. No significant increase of alkaline phosphatase (ALP) activity at 2days post-irradiation was found with a dose of 1.9Gy. However, ALP activity was significantly decreased after 7days. Using our model system, the results indicate that μCT imaging with doses as low as 1.9Gy, which is required to obtain a reasonable image quality, can induce irreparable damages on the cellular level

Statistics

Citations

Dimensions.ai Metrics
4 citations in Web of Science®
4 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

4 downloads since deposited on 12 Oct 2018
4 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:610 Medicine & health
Uncontrolled Keywords:alkaline phosphatase activity (ALP); cell survival; collagen; MC3T3-E1 pre-osteoblastic cells; micro-computed tomography (μCT); tissue engineering
Language:English
Date:1 January 2010
Deposited On:12 Oct 2018 14:32
Last Modified:24 Nov 2018 02:54
Publisher:De Gruyter
ISSN:0013-5585
OA Status:Green
Publisher DOI:https://doi.org/10.1515/bmt.2010.031
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicencegruyter101515bmt2010031 (Library Catalogue)

Download

Download PDF  'Effects of μCT radiation on tissue engineered bone-like constructs'.
Preview
Content: Published Version
Language: English
Filetype: PDF (Nationallizenz 142-005)
Size: 219kB
View at publisher