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Synthesis and regulation of leukaemia inhibitory factor in cultured bovine oviduct cells by hormones


Reinhart, K (1998). Synthesis and regulation of leukaemia inhibitory factor in cultured bovine oviduct cells by hormones. Molecular Human Reproduction, 4(3):301-308.

Abstract

Leukaemia inhibitory factor (LIF) is an essential factor for embryo implantation. Factors generated by the oviduct cells (epithelial cells and fibroblasts) create the microenvironment for fertilization and first embryo stage development. Hence, it is feasible that the oviduct cells also synthesize LIF to promote and condition the embryo for implantation in the uterus. In the present study, we investigated whether cultured bovine oviduct epithelial cells and fibroblasts synthesize LIF. LIF production was measured in the conditioned medium of oviduct epithelial cells and fibroblasts, using LIF enzyme-linked immunosorbent assay. Moreover, expression of LIF mRNA was confirmed by LIF reverse transcriptase-polymerase chain reaction in extracts of RNA from oviduct epithelial/fibroblast cells. Quantitatively similar amounts of LIF were detected in the culture medium of epithelial cells and fibroblasts. In cells cultured for 1-7 days, the levels of LIF in the medium increased in a time-dependent manner. As compared to untreated cells, treatment of cells with 17beta-oestradiol (1-100 ng/ ml), but not progesterone (1-100 ng/ml) and insulin (20 ng/ml), increased the levels of LIF in a concentration-dependent manner (P < 0.05). Similarly, tumour necrosis factor-alpha (100 ng/ml) significantly induced the levels of LIF. The effects of 17beta-oestradiol (50 ng/ml) on LIF synthesis were enhanced and not blocked in the presence of tamoxifen (1 microg/ml), an oestrogen receptor antagonist, suggesting that the stimulatory effects of 17beta-oestradiol on LIF synthesis are not receptor-mediated. In conclusion 17beta-oestradiol, but not progesterone, induces LIF synthesis by bovine oviduct epithelial cells and fibroblasts and this may play an important role in the biology of early embryo development. However, the exact pathophysiological role of LIF within the oviduct needs to be further investigated

Abstract

Leukaemia inhibitory factor (LIF) is an essential factor for embryo implantation. Factors generated by the oviduct cells (epithelial cells and fibroblasts) create the microenvironment for fertilization and first embryo stage development. Hence, it is feasible that the oviduct cells also synthesize LIF to promote and condition the embryo for implantation in the uterus. In the present study, we investigated whether cultured bovine oviduct epithelial cells and fibroblasts synthesize LIF. LIF production was measured in the conditioned medium of oviduct epithelial cells and fibroblasts, using LIF enzyme-linked immunosorbent assay. Moreover, expression of LIF mRNA was confirmed by LIF reverse transcriptase-polymerase chain reaction in extracts of RNA from oviduct epithelial/fibroblast cells. Quantitatively similar amounts of LIF were detected in the culture medium of epithelial cells and fibroblasts. In cells cultured for 1-7 days, the levels of LIF in the medium increased in a time-dependent manner. As compared to untreated cells, treatment of cells with 17beta-oestradiol (1-100 ng/ ml), but not progesterone (1-100 ng/ml) and insulin (20 ng/ml), increased the levels of LIF in a concentration-dependent manner (P < 0.05). Similarly, tumour necrosis factor-alpha (100 ng/ml) significantly induced the levels of LIF. The effects of 17beta-oestradiol (50 ng/ml) on LIF synthesis were enhanced and not blocked in the presence of tamoxifen (1 microg/ml), an oestrogen receptor antagonist, suggesting that the stimulatory effects of 17beta-oestradiol on LIF synthesis are not receptor-mediated. In conclusion 17beta-oestradiol, but not progesterone, induces LIF synthesis by bovine oviduct epithelial cells and fibroblasts and this may play an important role in the biology of early embryo development. However, the exact pathophysiological role of LIF within the oviduct needs to be further investigated

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Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:Unspecified
Scopus Subject Areas:Health Sciences > Reproductive Medicine
Health Sciences > Embryology
Life Sciences > Molecular Biology
Life Sciences > Genetics
Health Sciences > Obstetrics and Gynecology
Life Sciences > Developmental Biology
Life Sciences > Cell Biology
Language:English
Date:1 March 1998
Deposited On:25 Sep 2018 13:08
Last Modified:24 May 2020 04:40
Publisher:Oxford University Press
ISSN:1360-9947
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/molehr/4.3.301
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicenceoxford101093molehr43301 (Library Catalogue)

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