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Tissue engineering: A new approach in cardiovascular surgery; Seeding of human fibroblasts followed by human endothelial cells on resorbable mesh1


Zünd, Gregor; Hoerstrup, Simon P; Schoeberlein, Andreina; Lachat, Mario; Uhlschmid, Georg; Vogt, Paul R; Turina, Marko (1998). Tissue engineering: A new approach in cardiovascular surgery; Seeding of human fibroblasts followed by human endothelial cells on resorbable mesh1. European Journal of Cardio-Thoracic Surgery, 13(2):160-164.

Abstract

Objective: In tissue engineering the material properties of synthetic compounds are chosen to enable delivery of dissociated cells onto a scaffold in a manner that will result in in vitro formation of a new functional tissue. The seeding of human fibroblasts followed by human endothelial cells on resorbable mesh is a precondition of a successful creation of human tissues such as vessels or cardiac valves. Methods: Polymeric scaffolds (n=18) composed of polyglycolic acid (PGA) with a fiber diameter of 12-15 μm and a polymer density of 70 mg/ml were used as square sheets of 1×1×0.3 cm. Fibroblasts (passage 7) harvested from human foreskin were seeded (3.4×106) and cultured over a 3 week period on a PGA-mesh, followed by seeding of endothelial cells (passage 5, 2.8×106) harvested from human ascending aorta. Thereafter the new tissue was stained for HE, van Gieson, Trichrom-Masson, Factor VIII and CD 34 and proved by scanning electron microscopy. Results: Microscopic examination of the seeded mesh demonstrated that the human fibroblasts were attached to the polymeric fibers and had begun to spread out and divide. The scanning electron microscopic examination demonstrated a homogeneous scaffold resembling a solid sheet of tissue. The seeded endothelial cells formed a monolayer on the fibroblasts and no endothelial cell invasion or new formation of capillaris could be detected. Conclusions: These results are a first step to demonstrate that seeding of human fibroblasts and endothelial cells on PGA-mesh might be a feasible model to construct human tissues such as vessels or cardiac valves

Abstract

Objective: In tissue engineering the material properties of synthetic compounds are chosen to enable delivery of dissociated cells onto a scaffold in a manner that will result in in vitro formation of a new functional tissue. The seeding of human fibroblasts followed by human endothelial cells on resorbable mesh is a precondition of a successful creation of human tissues such as vessels or cardiac valves. Methods: Polymeric scaffolds (n=18) composed of polyglycolic acid (PGA) with a fiber diameter of 12-15 μm and a polymer density of 70 mg/ml were used as square sheets of 1×1×0.3 cm. Fibroblasts (passage 7) harvested from human foreskin were seeded (3.4×106) and cultured over a 3 week period on a PGA-mesh, followed by seeding of endothelial cells (passage 5, 2.8×106) harvested from human ascending aorta. Thereafter the new tissue was stained for HE, van Gieson, Trichrom-Masson, Factor VIII and CD 34 and proved by scanning electron microscopy. Results: Microscopic examination of the seeded mesh demonstrated that the human fibroblasts were attached to the polymeric fibers and had begun to spread out and divide. The scanning electron microscopic examination demonstrated a homogeneous scaffold resembling a solid sheet of tissue. The seeded endothelial cells formed a monolayer on the fibroblasts and no endothelial cell invasion or new formation of capillaris could be detected. Conclusions: These results are a first step to demonstrate that seeding of human fibroblasts and endothelial cells on PGA-mesh might be a feasible model to construct human tissues such as vessels or cardiac valves

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Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:1 February 1998
Deposited On:25 Sep 2018 13:10
Last Modified:24 Nov 2018 02:59
Publisher:Oxford University Press
ISSN:1010-7940
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/s1010-7940(97)00309-6
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicenceoxford101016S1010794097003096 (Library Catalogue)

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