Header

UZH-Logo

Maintenance Infos

Expression patterns of flagellin sensing 2 map to bacterial entry sites in plant shoots and roots


Beck, Martina; Wyrsch, Ines; Strutt, James; Wimalasekera, Rinukshi; Webb, Alex; Boller, Thomas; Robatzek, Silke (2014). Expression patterns of flagellin sensing 2 map to bacterial entry sites in plant shoots and roots. Journal of Experimental Botany, 65(22):6487-6498.

Abstract

Pathogens can colonize all plant organs and tissues. To prevent this, each cell must be capable of autonomously triggering defence. Therefore, it is generally assumed that primary sensors of the immune system are constitutively present. One major primary sensor against bacterial infection is the flagellin sensing 2 (FLS2) pattern recognition receptor (PRR). To gain insights into its expression pattern, the FLS2 promoter activity in β-glucuronidase (GUS) reporter lines was monitored. The data show that pFLS2::GUS activity is highest in cells and tissues vulnerable to bacterial entry and colonization, such as stomata, hydathodes, and lateral roots. GUS activity is also high in the vasculature and, by monitoring Ca(2+) responses in the vasculature, it was found that this tissue contributes to flg22-induced Ca(2+) burst. The FLS2 promoter is also regulated in a tissue- and cell type-specific manner and is responsive to hormones, damage, and biotic stresses. This results in stimulus-dependent expansion of the FLS2 expression domain. In summary, a tissue- and cell type-specific map of FLS2 expression has been created correlating with prominent entry sites and target tissues of plant bacterial pathogens.

Abstract

Pathogens can colonize all plant organs and tissues. To prevent this, each cell must be capable of autonomously triggering defence. Therefore, it is generally assumed that primary sensors of the immune system are constitutively present. One major primary sensor against bacterial infection is the flagellin sensing 2 (FLS2) pattern recognition receptor (PRR). To gain insights into its expression pattern, the FLS2 promoter activity in β-glucuronidase (GUS) reporter lines was monitored. The data show that pFLS2::GUS activity is highest in cells and tissues vulnerable to bacterial entry and colonization, such as stomata, hydathodes, and lateral roots. GUS activity is also high in the vasculature and, by monitoring Ca(2+) responses in the vasculature, it was found that this tissue contributes to flg22-induced Ca(2+) burst. The FLS2 promoter is also regulated in a tissue- and cell type-specific manner and is responsive to hormones, damage, and biotic stresses. This results in stimulus-dependent expansion of the FLS2 expression domain. In summary, a tissue- and cell type-specific map of FLS2 expression has been created correlating with prominent entry sites and target tissues of plant bacterial pathogens.

Statistics

Citations

Dimensions.ai Metrics
33 citations in Web of Science®
30 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

36 downloads since deposited on 31 Oct 2018
34 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:Unspecified
Uncontrolled Keywords:Bacteria; flagellin; flg22; pattern recognition receptor; promoter expression; stomata.
Language:English
Date:1 December 2014
Deposited On:31 Oct 2018 17:10
Last Modified:24 Sep 2019 23:41
Publisher:Oxford University Press
ISSN:0022-0957
OA Status:Green
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/jxb/eru366
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicenceoxford101093jxberu366 (Library Catalogue)
PubMed ID:25205577

Download

Green Open Access

Download PDF  'Expression patterns of flagellin sensing 2 map to bacterial entry sites in plant shoots and roots'.
Preview
Content: Published Version
Language: English
Filetype: PDF (Nationallizenz 142-005)
Size: 3MB
View at publisher