Header

UZH-Logo

Maintenance Infos

32P-Postlabeling of a DNA adduct derived from 4,4′-methylenedianiline, in the olfactory epithelium of rats exposed by inhalation to 4,4′-methylenediphenyl diisocyanate


Vock, E H; Hoymann, H-G; Heinrich, U; Lutz, W K (1996). 32P-Postlabeling of a DNA adduct derived from 4,4′-methylenedianiline, in the olfactory epithelium of rats exposed by inhalation to 4,4′-methylenediphenyl diisocyanate. Carcinogenesis, 17(5):1069-1073.

Abstract

Tissues obtained from female Wistar rats exposed to a 0.9 μm aerosol of 4,4′-methylenediphenyl diisocyanate (MDI) for 17 h per day, 5 days per week, for one year, at levels of 0, 0.3, 0.7 and 2.0 mg/m3, were analyzed for DNA adducts. A 32P-postlabeling method was used to detect (i). adducts formed by the reaction of the isocyanate group(s) of MDI with DNA; and a 32P-postlabeling method was adapted to detect (ii), a DNA adduct formed by 4,4′-methylenedianiline (MDA), a hydrolysis/decarboxylation product of MDI. In the lung, neither isocyanate adducts nor the arylamine adduct were detectable. The same negative result was seen in the liver, the bladder, the kidney, the respiratory epithelium and in peripheral lymphocytes. In the olfactory epithelium, on the other hand, the aryl-amine-derived DNA adduct was detected, at the very low levels of 5, 9 and 10 adduct-nucleotides per 1010 nucleotides, for the three dose groups, respectively. The adduct co-chromatographed with the one formed in the liver of rats after oral gavage of MDA. The results are discussed in terms of the importance of genotoxic versus nongenotoxic aspects of carcinogenesis

Abstract

Tissues obtained from female Wistar rats exposed to a 0.9 μm aerosol of 4,4′-methylenediphenyl diisocyanate (MDI) for 17 h per day, 5 days per week, for one year, at levels of 0, 0.3, 0.7 and 2.0 mg/m3, were analyzed for DNA adducts. A 32P-postlabeling method was used to detect (i). adducts formed by the reaction of the isocyanate group(s) of MDI with DNA; and a 32P-postlabeling method was adapted to detect (ii), a DNA adduct formed by 4,4′-methylenedianiline (MDA), a hydrolysis/decarboxylation product of MDI. In the lung, neither isocyanate adducts nor the arylamine adduct were detectable. The same negative result was seen in the liver, the bladder, the kidney, the respiratory epithelium and in peripheral lymphocytes. In the olfactory epithelium, on the other hand, the aryl-amine-derived DNA adduct was detected, at the very low levels of 5, 9 and 10 adduct-nucleotides per 1010 nucleotides, for the three dose groups, respectively. The adduct co-chromatographed with the one formed in the liver of rats after oral gavage of MDA. The results are discussed in terms of the importance of genotoxic versus nongenotoxic aspects of carcinogenesis

Statistics

Citations

Dimensions.ai Metrics
21 citations in Web of Science®
26 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

6 downloads since deposited on 11 Oct 2018
6 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:1 January 1996
Deposited On:11 Oct 2018 14:29
Last Modified:24 Nov 2018 03:02
Publisher:Oxford University Press
ISSN:0143-3334
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/carcin/17.5.1069
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicenceoxford101093carcin1751069 (Library Catalogue)

Download

Download PDF  '32P-Postlabeling of a DNA adduct derived from 4,4′-methylenedianiline, in the olfactory epithelium of rats exposed by inhalation to 4,4′-methylenediphenyl diisocyanate'.
Preview
Content: Published Version
Language: English
Filetype: PDF (Nationallizenz 142-005)
Size: 2MB
View at publisher