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Detection of Capsular Polysaccharide in Serum for the Diagnosis of Pneumococcal Pneumonia: Clinical and Experimental Evaluation


Schaffner, A; Michel-Harder, C; Yeginsoy, S (1991). Detection of Capsular Polysaccharide in Serum for the Diagnosis of Pneumococcal Pneumonia: Clinical and Experimental Evaluation. Journal of Infectious Diseases, 163(5):1094-1102.

Abstract

To improve diagnostic options for pneumococcal pneumonia, an ELISA system was developed that can detect ⩽6 ng/ml capsular polysaccharide in serum. The test waslimited to 39 serotypes causing >95% of pneumococcal infections. In clinical evaluation the test identified 14 of 15 cases (missing one serotype not included). No false-positive reaction occurred. However, the duration and level of antigenemia were variable (⩾500-2.5 ng/ml) and seemed not to depend solely on the severity of infection. Therefore, the question of whether the extent of antigenemia was determined by a serotype-dependent variation in the elimination rates of polysaccharides was investigated. Clearance rates for 12 serotypes varied in rabbits and rats by a factor of >250. This remarkable variability appeared to affect the extent of clinical antigenemia. Thus, only very sensitive systems can detect circulating antigen from rapidly cleared polysaccharide serotypes. Furthermore, the question arises whether slow polysaccharide clearance contributes to the virulence of some pneumococcal serotypes

Abstract

To improve diagnostic options for pneumococcal pneumonia, an ELISA system was developed that can detect ⩽6 ng/ml capsular polysaccharide in serum. The test waslimited to 39 serotypes causing >95% of pneumococcal infections. In clinical evaluation the test identified 14 of 15 cases (missing one serotype not included). No false-positive reaction occurred. However, the duration and level of antigenemia were variable (⩾500-2.5 ng/ml) and seemed not to depend solely on the severity of infection. Therefore, the question of whether the extent of antigenemia was determined by a serotype-dependent variation in the elimination rates of polysaccharides was investigated. Clearance rates for 12 serotypes varied in rabbits and rats by a factor of >250. This remarkable variability appeared to affect the extent of clinical antigenemia. Thus, only very sensitive systems can detect circulating antigen from rapidly cleared polysaccharide serotypes. Furthermore, the question arises whether slow polysaccharide clearance contributes to the virulence of some pneumococcal serotypes

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Health Sciences > Immunology and Allergy
Health Sciences > Infectious Diseases
Language:English
Date:1 May 1991
Deposited On:16 Oct 2018 15:18
Last Modified:15 Apr 2021 14:50
Publisher:Oxford University Press
ISSN:0022-1899
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/infdis/163.5.1094

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