Header

UZH-Logo

Maintenance Infos

Structure-dependent bypass of DNA interstrand crosslinks by translesion synthesis polymerases


Ho, The Vinh; Guainazzi, Angelo; Derkunt, Semsi Burak; Enoiu, Milica; Schärer, Orlando D (2011). Structure-dependent bypass of DNA interstrand crosslinks by translesion synthesis polymerases. Nucleic Acids Research, 39(17):7455-7464.

Abstract

DNA interstrand crosslinks (ICLs), inhibit DNA metabolism by covalently linking two strands of DNA and are formed by antitumor agents such as cisplatin and nitrogen mustards. Multiple complex repair pathways of ICLs exist in humans that share translesion synthesis (TLS) past a partially processed ICL as a common step. We have generated site-specific major groove ICLs and studied the ability of Y-family polymerases and Pol ζ to bypass ICLs that induce different degrees of distortion in DNA. Two main factors influenced the efficiency of ICL bypass: the length of the dsDNA flanking the ICL and the length of the crosslink bridging two bases. Our study shows that ICLs can readily be bypassed by TLS polymerases if they are appropriately processed and that the structure of the ICL influences which polymerases are able to read through it

Abstract

DNA interstrand crosslinks (ICLs), inhibit DNA metabolism by covalently linking two strands of DNA and are formed by antitumor agents such as cisplatin and nitrogen mustards. Multiple complex repair pathways of ICLs exist in humans that share translesion synthesis (TLS) past a partially processed ICL as a common step. We have generated site-specific major groove ICLs and studied the ability of Y-family polymerases and Pol ζ to bypass ICLs that induce different degrees of distortion in DNA. Two main factors influenced the efficiency of ICL bypass: the length of the dsDNA flanking the ICL and the length of the crosslink bridging two bases. Our study shows that ICLs can readily be bypassed by TLS polymerases if they are appropriately processed and that the structure of the ICL influences which polymerases are able to read through it

Statistics

Citations

Dimensions.ai Metrics
49 citations in Web of Science®
46 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

46 downloads since deposited on 08 Nov 2018
45 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:1 September 2011
Deposited On:08 Nov 2018 17:11
Last Modified:24 Sep 2019 23:42
Publisher:Oxford University Press
ISSN:0305-1048
OA Status:Gold
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/nar/gkr448
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicenceoxford101093nargkr448 (Library Catalogue)
PubMed ID:21666254

Download

Gold Open Access

Download PDF  'Structure-dependent bypass of DNA interstrand crosslinks by translesion synthesis polymerases'.
Preview
Content: Published Version
Language: English
Filetype: PDF (Nationallizenz 142-005)
Size: 5MB
View at publisher