Header

UZH-Logo

Maintenance Infos

Proper layering is important for precisely timed activation of hippocampal mossy cells


Kowalski, Janina; Geuting, Markus; Paul, Sebastian; Dieni, Sandra; Laurens, Jean; Zhao, Shanting; Drakew, Alexander; Haas, Carola A; Frotscher, Michael; Vida, Imre (2010). Proper layering is important for precisely timed activation of hippocampal mossy cells. Cerebral Cortex, 20(9):2043-2054.

Abstract

The mammalian cortex exhibits a laminated structure that may underlie optimal synaptic connectivity and support temporally precise activation of neurons. In ‘reeler' mice, the lack of the extracellular matrix protein Reelin leads to abnormal positioning of cortical neurons and disrupted layering. To address how these structural changes impact neuronal function, we combined electrophysiological and neuroanatomical techniques to investigate the synaptic activation of hippocampal mossy cells (MCs), the cell type that integrates the output of dentate gyrus granule cells (GCs). While somatodendritic domains of wild-type (WT) MCs were confined to the hilus, the somata and dendrites of reeler MCs were often found in the molecular layer, where the perforant path (PP) terminates. Most reeler MCs received aberrant monosynaptic excitatory input from the PP, whereas the disynaptic input to MCs via GCs was decreased and inhibition was increased. In contrast to the uniform disynaptic discharge of WT MCs, many reeler cells discharged with short, monosynaptic latencies, while others fired with long latencies over a broad temporal window in response to PP activation. Thus, disturbed lamination results in aberrant synaptic connectivity and altered timing of action potential generation. These results highlight the importance of a layered cortical structure for information processing

Abstract

The mammalian cortex exhibits a laminated structure that may underlie optimal synaptic connectivity and support temporally precise activation of neurons. In ‘reeler' mice, the lack of the extracellular matrix protein Reelin leads to abnormal positioning of cortical neurons and disrupted layering. To address how these structural changes impact neuronal function, we combined electrophysiological and neuroanatomical techniques to investigate the synaptic activation of hippocampal mossy cells (MCs), the cell type that integrates the output of dentate gyrus granule cells (GCs). While somatodendritic domains of wild-type (WT) MCs were confined to the hilus, the somata and dendrites of reeler MCs were often found in the molecular layer, where the perforant path (PP) terminates. Most reeler MCs received aberrant monosynaptic excitatory input from the PP, whereas the disynaptic input to MCs via GCs was decreased and inhibition was increased. In contrast to the uniform disynaptic discharge of WT MCs, many reeler cells discharged with short, monosynaptic latencies, while others fired with long latencies over a broad temporal window in response to PP activation. Thus, disturbed lamination results in aberrant synaptic connectivity and altered timing of action potential generation. These results highlight the importance of a layered cortical structure for information processing

Statistics

Citations

Dimensions.ai Metrics
9 citations in Web of Science®
11 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

31 downloads since deposited on 15 Nov 2018
29 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:Unspecified
Language:English
Date:1 September 2010
Deposited On:15 Nov 2018 14:38
Last Modified:24 Sep 2019 23:43
Publisher:Oxford University Press
ISSN:1047-3211
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1093/cercor/bhp267
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicenceoxford101093cercorbhp267 (Library Catalogue)
PubMed ID:20053714

Download

Green Open Access

Download PDF  'Proper layering is important for precisely timed activation of hippocampal mossy cells'.
Preview
Content: Published Version
Language: English
Filetype: PDF (Nationallizenz 142-005)
Size: 1MB
View at publisher