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Hemoglobinometry as a method for measuring blood meal sizes of Mosquitoes (Diptera: Culicidae)1


Briegel, Hans; Lea, Arden O; Klowden, Marc J (1979). Hemoglobinometry as a method for measuring blood meal sizes of Mosquitoes (Diptera: Culicidae)1. Journal of Medical Entomology, 15(3):235-238.

Abstract

A standardized clinical method for measuring hemoglobin has been applied to the measurement of the quantity of blood ingested by Aedes aegypti feeding on a vertebrate host. The midgut of a blood-fed mosquito is added to a reagent which converts all hemoglobin to hemiglobincyanide (HiCN) which can be read spectrophotometrically. A sample of the host's blood is required as a standard. We established the validity of this method by comparing hemoglobin determinations with the size of blood meals measured by weight as well as with blood meals administered as enemas. The method is independent of urination and digestion by the mosquito for at least 12 h after feeding (at 27°C) and can be applied to females feeding on an unrestrained host during crepuscular or dark periods. A disadvantage of this method is that females must be killed in order to determine the volume of blood they ingest. Once converted to hemiglobincyanide, the blood meal can be frozen and stored for later analysis

Abstract

A standardized clinical method for measuring hemoglobin has been applied to the measurement of the quantity of blood ingested by Aedes aegypti feeding on a vertebrate host. The midgut of a blood-fed mosquito is added to a reagent which converts all hemoglobin to hemiglobincyanide (HiCN) which can be read spectrophotometrically. A sample of the host's blood is required as a standard. We established the validity of this method by comparing hemoglobin determinations with the size of blood meals measured by weight as well as with blood meals administered as enemas. The method is independent of urination and digestion by the mosquito for at least 12 h after feeding (at 27°C) and can be applied to females feeding on an unrestrained host during crepuscular or dark periods. A disadvantage of this method is that females must be killed in order to determine the volume of blood they ingest. Once converted to hemiglobincyanide, the blood meal can be frozen and stored for later analysis

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
600 Technology
Scopus Subject Areas:Life Sciences > Parasitology
Health Sciences > General Veterinary
Life Sciences > Insect Science
Health Sciences > Infectious Diseases
Language:English
Date:23 March 1979
Deposited On:15 Nov 2018 15:01
Last Modified:31 Jul 2020 02:27
Publisher:Oxford University Press
ISSN:0022-2585
OA Status:Green
Publisher DOI:https://doi.org/10.1093/jmedent/15.3.235
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicenceoxford101093jmedent153235 (Library Catalogue)

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