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Equal Amounts of Intracellular and Virion‐Enclosed Hepatitis C Virus RNA Are Associated with Peripheral‐Blood Mononuclear Cells In Vivo


Kaiser, Philipp; Niederöst, Barbara; Joos, Beda; von Wyl, Viktor; Opravil, Milos; Weber, Rainer; Günthard, Huldrych F; Fischer, Marek (2006). Equal Amounts of Intracellular and Virion‐Enclosed Hepatitis C Virus RNA Are Associated with Peripheral‐Blood Mononuclear Cells In Vivo. Journal of Infectious Diseases, 194(12):1713-1723.

Abstract

Background. Hepatitis C virus (HCV) replicating in peripheral‐blood mononuclear cells (PBMCs) may represent an extrahepatic viral reservoir. Quantitation of HCV RNA with regard to its subcellular distribution and longitudinal course is needed for better understanding of the largely unexplored in vivo dynamics and potential pathogenetic significance of HCV in PBMCs. Methods. Plasma and PBMCs from 30 patients coinfected with HCV and human immunodeficiency virus were evaluated in cross‐sectional and longitudinal analyses, for up to 40 months. Differential extraction of virion‐enclosed HCV RNA associated with cells was performed in parallel with extraction of total cellular HCV RNA. HCV RNA of either orientation was quantified by real‐time polymerase chain reaction. Results. HCV RNA was detected only in PBMCs from patients with viremia and at relatively stable quantities over time. Intracellular HCV RNA corresponding to ∼60% of total cellular HCV RNA was strongly correlated with virion‐enclosed HCV RNA but was only weakly associated with viral loads in plasma. In contrast, the ratio of HCV RNA load in PBMCs versus that in plasma was patient specific and stable over time. Conclusions. The substantial and patient‐specific amounts of intracellular HCV RNA found by the present study support a concept of low‐level replication in PBMCs. There was no evidence for persistent HCV infection in PBMCs after clearance of viremia in plasma

Abstract

Background. Hepatitis C virus (HCV) replicating in peripheral‐blood mononuclear cells (PBMCs) may represent an extrahepatic viral reservoir. Quantitation of HCV RNA with regard to its subcellular distribution and longitudinal course is needed for better understanding of the largely unexplored in vivo dynamics and potential pathogenetic significance of HCV in PBMCs. Methods. Plasma and PBMCs from 30 patients coinfected with HCV and human immunodeficiency virus were evaluated in cross‐sectional and longitudinal analyses, for up to 40 months. Differential extraction of virion‐enclosed HCV RNA associated with cells was performed in parallel with extraction of total cellular HCV RNA. HCV RNA of either orientation was quantified by real‐time polymerase chain reaction. Results. HCV RNA was detected only in PBMCs from patients with viremia and at relatively stable quantities over time. Intracellular HCV RNA corresponding to ∼60% of total cellular HCV RNA was strongly correlated with virion‐enclosed HCV RNA but was only weakly associated with viral loads in plasma. In contrast, the ratio of HCV RNA load in PBMCs versus that in plasma was patient specific and stable over time. Conclusions. The substantial and patient‐specific amounts of intracellular HCV RNA found by the present study support a concept of low‐level replication in PBMCs. There was no evidence for persistent HCV infection in PBMCs after clearance of viremia in plasma

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Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:15 December 2006
Deposited On:02 Nov 2018 07:26
Last Modified:24 Sep 2019 23:43
Publisher:Oxford University Press
ISSN:0022-1899
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1086/508431
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicenceoxford101086508431 (Library Catalogue)

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