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CARD In Situ hybridization: sights and signals


Speel, Ernst J M; Komminoth, Paul (1999). CARD In Situ hybridization: sights and signals. Endocrine Pathology, 10(3):193-198.

Abstract

During the last decade, several strategies have been developed to improve the detection sensitivity ofin situ hybridization (ISH) by amplification of either target nucleic acid sequences prior to ISH (e.g.,in situ PCR), or the detection signals after the hybridization procedures (signal amplification). Here we outline the principles of tyramide signal amplification using the catalyzed reporter deposition (CARD) technique, summarize applications as well as possible limitations of CARD ISH, and discuss some future directions ofin situ nucleic acid detection using this amplification strategy

Abstract

During the last decade, several strategies have been developed to improve the detection sensitivity ofin situ hybridization (ISH) by amplification of either target nucleic acid sequences prior to ISH (e.g.,in situ PCR), or the detection signals after the hybridization procedures (signal amplification). Here we outline the principles of tyramide signal amplification using the catalyzed reporter deposition (CARD) technique, summarize applications as well as possible limitations of CARD ISH, and discuss some future directions ofin situ nucleic acid detection using this amplification strategy

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Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:Unspecified
Language:English
Date:1 September 1999
Deposited On:28 Nov 2018 16:16
Last Modified:28 Nov 2018 16:16
Publisher:Springer
ISSN:1046-3976
OA Status:Green
Publisher DOI:https://doi.org/10.1007/bf02738880
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicencespringer101007BF02738880 (Library Catalogue)
PubMed ID:12114699

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