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Expression and Activity Patterns of Nitric Oxide Synthases and Antioxidant Enzymes Reveal a Substantial Heterogeneity Between Cardiac and Vascular Aging in the Rat


van der Loo, Bernd; Bachschmid, Markus; Labugger, Ralf; Schildknecht, Stefan; Kilo, Juliane; Hahn, Raphael; Palacios-Callender, Miriam; Lüscher, Thomas F (2005). Expression and Activity Patterns of Nitric Oxide Synthases and Antioxidant Enzymes Reveal a Substantial Heterogeneity Between Cardiac and Vascular Aging in the Rat. Biogerontology, 6(5):325-334.

Abstract

We investigated the effects of aging and ischemia-reperfusion (I/R) injury on the expression and activity of nitric oxide (•NO) synthases and superoxide dismutase (SOD) isoforms. To this end we perfused excised hearts from young (6months old) and old (31-34months old) rats according to the Langendorff technique. The isolated hearts were, after baseline perfusion for 30min, either subjected to 20min of global no-flow ischemia followed by 40min of reperfusion or were control-perfused (60min normoxic perfusion). Both MnSOD and Cu,ZnSOD expression remained unchanged with increasing age and remained unaltered by I/R. However, SOD activity decreased from 7.55 ± 0.1U/mg protein in young hearts to 5.94 ± 0.44 in old hearts (P<0.05). Furthermore, I/R led to a further decrease in enzyme activity (to 6.35 ± 0.41U/mg protein; P<0.05) in myocardium of young, but not in that of old animals. No changes in myocardial protein-bound 3-nitrotyrosine levels could be detected. Endothelial NOS (eNOS) expression and activity remained unchanged in aged left ventricles, irrespective of I/R injury. This was in steep contrast to peripheral (renal and femoral) arteries obtained from the same animals where a marked age-associated increase of eNOS protein expression could be demonstrated. Inducible NOS expression was undetectable either in the peripheral arteries or in the left ventricle, irrespective of age. In particular when associated with an acute pathology, which is furthermore limited to a certain time frame, changes in the aged myocardium with respect to enzymes crucially involved in maintaining the redox homeostasis, seem to be much less pronounced or even absent compared to the vascular aging process. This may point to heterogeneity in the molecular regulation of the cardiovascular aging process

Abstract

We investigated the effects of aging and ischemia-reperfusion (I/R) injury on the expression and activity of nitric oxide (•NO) synthases and superoxide dismutase (SOD) isoforms. To this end we perfused excised hearts from young (6months old) and old (31-34months old) rats according to the Langendorff technique. The isolated hearts were, after baseline perfusion for 30min, either subjected to 20min of global no-flow ischemia followed by 40min of reperfusion or were control-perfused (60min normoxic perfusion). Both MnSOD and Cu,ZnSOD expression remained unchanged with increasing age and remained unaltered by I/R. However, SOD activity decreased from 7.55 ± 0.1U/mg protein in young hearts to 5.94 ± 0.44 in old hearts (P<0.05). Furthermore, I/R led to a further decrease in enzyme activity (to 6.35 ± 0.41U/mg protein; P<0.05) in myocardium of young, but not in that of old animals. No changes in myocardial protein-bound 3-nitrotyrosine levels could be detected. Endothelial NOS (eNOS) expression and activity remained unchanged in aged left ventricles, irrespective of I/R injury. This was in steep contrast to peripheral (renal and femoral) arteries obtained from the same animals where a marked age-associated increase of eNOS protein expression could be demonstrated. Inducible NOS expression was undetectable either in the peripheral arteries or in the left ventricle, irrespective of age. In particular when associated with an acute pathology, which is furthermore limited to a certain time frame, changes in the aged myocardium with respect to enzymes crucially involved in maintaining the redox homeostasis, seem to be much less pronounced or even absent compared to the vascular aging process. This may point to heterogeneity in the molecular regulation of the cardiovascular aging process

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Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:Unspecified
Scopus Subject Areas:Life Sciences > Aging
Health Sciences > Gerontology
Health Sciences > Geriatrics and Gerontology
Language:English
Date:1 November 2005
Deposited On:23 Oct 2018 15:09
Last Modified:31 Jul 2020 02:36
Publisher:Springer
ISSN:1389-5729
OA Status:Green
Publisher DOI:https://doi.org/10.1007/s10522-005-4807-1
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicencespringer101007s1052200548071 (Library Catalogue)

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