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FRET Studies of the Interaction of Dimeric Cyanine Dyes with DNA


Laib, Stephan; Seeger, Stefan (2004). FRET Studies of the Interaction of Dimeric Cyanine Dyes with DNA. Journal of Fluorescence, 14(2):187-191.

Abstract

Fluorescence Resonance Energy Transfer (FRET) is a powerful tool to determine distances between chromophores bound to macromolecules, since the efficiency of the energy transfer from an initially excited donor to an acceptor strongly depends on the distance between the two dye molecules. The structure of the noncovalent complex of double-strand DNA (dsDNA) with thiazol orange dimers (TOTO) allows FRET analysis of two intercalated chromophores. By intercalation of two different TOTO dyes we observe an energy transfer from TOTO-1 as donor and TOTO-3 as acceptor. In this manner we are able to determine the mean distance between two proximate TOTO molecules bound to dsDNA. Thus the maximum number of binding positions for this type of intercalation dyes in the dsDNA can be obtained. Furthermore the dependency of the acceptor emission on the donor concentration is analysed. The emission of TOTO-3 reaches a maximum when the acceptor-to-donor ratio is 1:10

Abstract

Fluorescence Resonance Energy Transfer (FRET) is a powerful tool to determine distances between chromophores bound to macromolecules, since the efficiency of the energy transfer from an initially excited donor to an acceptor strongly depends on the distance between the two dye molecules. The structure of the noncovalent complex of double-strand DNA (dsDNA) with thiazol orange dimers (TOTO) allows FRET analysis of two intercalated chromophores. By intercalation of two different TOTO dyes we observe an energy transfer from TOTO-1 as donor and TOTO-3 as acceptor. In this manner we are able to determine the mean distance between two proximate TOTO molecules bound to dsDNA. Thus the maximum number of binding positions for this type of intercalation dyes in the dsDNA can be obtained. Furthermore the dependency of the acceptor emission on the donor concentration is analysed. The emission of TOTO-3 reaches a maximum when the acceptor-to-donor ratio is 1:10

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Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:Unspecified
Scopus Subject Areas:Life Sciences > Biochemistry
Social Sciences & Humanities > Clinical Psychology
Social Sciences & Humanities > Social Sciences (miscellaneous)
Social Sciences & Humanities > Sociology and Political Science
Physical Sciences > Spectroscopy
Life Sciences > Clinical Biochemistry
Social Sciences & Humanities > Law
Language:English
Date:1 March 2004
Deposited On:19 Oct 2018 12:09
Last Modified:31 Jul 2020 02:46
Publisher:Springer
ISSN:1053-0509
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1023/b:jofl.0000016290.34070.ee
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicencespringer101023BJOFL000001629034070ee (Library Catalogue)

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