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Hypertonicity induced apoptosis in HL-60 cells in the presence of intracellular potassium


Ghosh, Arko; Keng, Peter C; Knauf, Philip A (2007). Hypertonicity induced apoptosis in HL-60 cells in the presence of intracellular potassium. Apoptosis, 12(7):1281-1288.

Abstract

Cell shrinkage is a hallmark of apoptosis. Potassium efflux, which is involved in cell shrinkage, has been previously described as an essential event of apoptosis. This study was designed to address the importance of potassium efflux in hypertonicity (450 mOsm and 600 mOsm) induced apoptosis. We initiated apoptosis in HL-60 cells in hypertonic medium consisting of either high concentrations of NaCl, mannitol or KCl. Apoptotic activity was evaluated based on the DNA content of the cells, annexin-V staining and calcium content. Apoptosis was initiated in hypertonic conditions consisting of high intracellular K+. We demonstrate that apoptosis can occur in the presence of high intracellular potassium contrary to previous predictions

Abstract

Cell shrinkage is a hallmark of apoptosis. Potassium efflux, which is involved in cell shrinkage, has been previously described as an essential event of apoptosis. This study was designed to address the importance of potassium efflux in hypertonicity (450 mOsm and 600 mOsm) induced apoptosis. We initiated apoptosis in HL-60 cells in hypertonic medium consisting of either high concentrations of NaCl, mannitol or KCl. Apoptotic activity was evaluated based on the DNA content of the cells, annexin-V staining and calcium content. Apoptosis was initiated in hypertonic conditions consisting of high intracellular K+. We demonstrate that apoptosis can occur in the presence of high intracellular potassium contrary to previous predictions

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Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:8 June 2007
Deposited On:03 Jul 2019 13:18
Last Modified:24 Sep 2019 23:47
Publisher:Springer
ISSN:1360-8185
OA Status:Green
Publisher DOI:https://doi.org/10.1007/s10495-007-0054-z
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicencespringer101007s104950070054z (Library Catalogue)
PubMed ID:17333319

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