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Peripheral blood levels of matrix metalloproteinase-9 predict lesion volume in acute stroke


Ulrich, Nils H; Dehmel, T; Wittsack, H J; Kieseier, B C; Seitz, R J (2013). Peripheral blood levels of matrix metalloproteinase-9 predict lesion volume in acute stroke. Neurological Sciences, 34(3):379-382.

Abstract

Matrix metalloproteinases (MMPs) have been implicated to play an important role in the destruction of the extracellular matrix in diseases of the central nervous system. This study investigated whether the expression of one of these proteases, MMP-9 in blood, is related to the size of human brain infarcts assessed with magnetic resonance imaging. Consecutively, twenty-one acute stroke patients were included prospectively into our study. In blood samples drawn within 24h after onset, MMP-9 RNA-expression and proteolytic-activity were analyzed by quantitative polymerase chain reaction and gelatin zymography, respectively. The ischemic lesion volumes in time to peak perfusion maps and diffusion weighted imaging were measured morphometrically. RNA-expression levels of MMP-9 in peripheral blood mononuclear cells (PBMCs) correlated with the brain infarct lesion (TTP-delay 4s, r=−0.61, p=0.007; TTP-delay 6s: r=−0.58, p=0.012; DWI r=−0.47; p=0.047). Our preliminary results demonstrate that MMP-9 RNA is upregulated in PBMCs in proportion to ischemia. These findings suggest that MMP-9 might contribute to the manifestation of ischemic brain damage. Since MMP-9 is upregulated in acute ischemia inhibition of MMP-9 may represent a complementary treatment target in acute stroke therapy

Abstract

Matrix metalloproteinases (MMPs) have been implicated to play an important role in the destruction of the extracellular matrix in diseases of the central nervous system. This study investigated whether the expression of one of these proteases, MMP-9 in blood, is related to the size of human brain infarcts assessed with magnetic resonance imaging. Consecutively, twenty-one acute stroke patients were included prospectively into our study. In blood samples drawn within 24h after onset, MMP-9 RNA-expression and proteolytic-activity were analyzed by quantitative polymerase chain reaction and gelatin zymography, respectively. The ischemic lesion volumes in time to peak perfusion maps and diffusion weighted imaging were measured morphometrically. RNA-expression levels of MMP-9 in peripheral blood mononuclear cells (PBMCs) correlated with the brain infarct lesion (TTP-delay 4s, r=−0.61, p=0.007; TTP-delay 6s: r=−0.58, p=0.012; DWI r=−0.47; p=0.047). Our preliminary results demonstrate that MMP-9 RNA is upregulated in PBMCs in proportion to ischemia. These findings suggest that MMP-9 might contribute to the manifestation of ischemic brain damage. Since MMP-9 is upregulated in acute ischemia inhibition of MMP-9 may represent a complementary treatment target in acute stroke therapy

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:National licences > 142-005
Dewey Decimal Classification:Unspecified
Language:English
Date:1 March 2013
Deposited On:04 Jul 2019 15:13
Last Modified:24 Sep 2019 23:47
Publisher:Springer
ISSN:1590-1874
OA Status:Green
Publisher DOI:https://doi.org/10.1007/s10072-012-0999-8
Related URLs:https://www.swissbib.ch/Search/Results?lookfor=nationallicencespringer101007s1007201209998 (Library Catalogue)
PubMed ID:22395947

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